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Rambler's Top100

Beer and beverages №2/2024



TOPIC OF THE ISSUE: MODERN TECHNOLOGIES FOR THE PRODUCTION OF BEER AND BEVERAGES

Kobelev K.V., Gribkova I.N., Borisenko O.A.The Alcohol's Influence Studies on the Hop Granules Organic Compound Group's Extraction

P. 4-8 DOI: 10.52653/PIN.2024.02.02

Key words
hop products, physical treatment, organic extragents, EHA-water, bitter resins, phenolic compounds, nitrogen compounds

Abstract
The article is devoted to the question of studying methods for hop organic compounds groups isolating. The purpose of the work was to study the influence of physicochemical methods for extracting hop organic compounds groups (bitter resins, polyphenols and nitrogen ones), using the example of the Cascade variety, with alcohols with different numbers of hydroxyl groups. The research was carried out using generally accepted photoelectrocolorimetric methods of analysis, as well as methods of statistical data processing. The authors showed that ethanol, propylene glycol and glycerin under different conditions of physicochemical treatment contributed to the extraction of bitter resins in an amount of about 30.8%, and alkaline reagents influenced the increase in the yield of total resins. The yield of polyphenolic compounds in the presence of ethanol without ultrasound increased after 20 min of treatment by 2.2 times in the control and by 2.7 times when using ethanol and KOH. The use of alkaline reagents (catalyte and KOH) and ethanol made it possible to increase the yield of phenolic compounds by 1.4 and 2 times, respectively, compared to the extracts obtained without ultrasound after 10 min of treatment. Increasing the duration of treatment in the presence of ultrasound and alkaline reagents led to a decrease in the yield of polyphenolic compounds. The use of propylene glycol also showed a decrease in the content of polyphenols without ultrasound in 20 minutes, and the most effective way to isolate polyphenols was a combination of ultrasound, propylene glycol and KOH, which in 20 minutes increased the yield of compounds by 2 times compared to treatment without ultrasound. Extraction with glycerol makes it possible to increase the yield of phenolic compounds by 1.5 times, in combination with KOH - by 2.8 times compared to 10 min of treatment without ultrasound. Ultrasound in combination with KOH and 10 min of treatment increases the yield of polyphenols by 6.6 times compared to treatment without ultrasound. The results regarding the extraction of nitrogenous compounds are presented. The effectiveness of using ultrasound and ethanol, catalyte and 10 min of treatment has been shown. At the same time, the yield of nitrogenous substances increased 3 times compared to treatment without ultrasound. It is noted that the release of nitrogenous compounds depends on the structure of the alcohol and extraction conditions. It has been shown that there is a moderate relationship (0.5<=r<=0.7) between the content of total polyphenols and bitter resins in the structure of hops, which depends on the structure of the extractant. Statistical analysis showed that there is a relationship in the structure of hops between bitter resins, phenolic and nitrogen compounds.

References
1. Dušek M, Jandovská V, Kalachová K. Comparative study of three sample preparation methods for multi-residue extraction of pesticide residues in hop samples. Food Analytical Methods. 2020;13:503–515. https://doi.org/10.1007/s12161-019-01658-6.
2. Rutnik K, Ocvirk M, Košir IJ. Changes in Hop (Humulus lupulus L.) Oil Content and Composition during Long-Term Storage under Different Conditions. Foods. 2022;11(19):3089. https://doi.org/10.3390/foods11193089.
3. Narciss L. The brewering. Vol. II. The wort production technics. Мoscow: NPO «Elevar», 1999. 370 p. (In Russ.).
4. Lafontaine SR. Investigating the Quality dynamics of American aroma hops intended for dry-hopping beer. Thesis for: PhD in Food Science and Technology. USA, Oregon State University, 2019. 245 p. URL: https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/kk91fr75p.
5. Kirkpatrick K. Investigating Hop Enzymes. Masters Thesis in Food Science and Technology. USA, Oregon State University, 2018. 125 p. URL: https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/41687p49q.
6. Sun S, Wang X, Yuan A, Liu J, Li Z, Xie D, [et al.]. Chemical constituents and bioactivities of hops (Humulus lupulus L.) and their effects on beer-related microorganisms. Food and Energy Security. 2022;11:e367. https://doi.org/10.1002/fes3.367.
7. Lordan R, O’Keeffe E, Tsoupras A, Zabetakis I. Total, neutral, and polar lipids of brewing ingredients, by-products and beer: evaluation of antithrombotic activities. Foods. 2019;8(5):171. https://doi.org/10.3390/foods8050171.
8. Zakharov МА, Zakharova VА., Gribkova IN. Transfer of hop nutraceuticals from processed brewer’s grains and their qualitative composition. Pivo i napitki = Beer and beverages. 2023;(4):4–9. https://doi.org/10.52653/PIN.2023.04.003. (In Russ.).
9. Castro P, Luz E, Moreira G. Dataset for Hop varieties classification. Data in Brief. 2021;38:107312. https://doi.org/10.1016/j.dib.2021.107312.
10. Yamauchi H. Hop-variety Identification Using First- and Second-generation Sequencing. In book: Next Generation Sequencing — Advances, Applications and Challenges. 2016. 464 p. https://doi.org/10.5772/61673.
11. Latyipova GМ, Ayupova GV, Bubenchikova VN, Galimova DF, Batyirova ED, Shafikova SF. Research on the content of bitter acids in common hop raw materials. The actual medicines problems. 2012; (10-2(129)):65–69. (In Russ.).
12. GOST 34798–2021. The brewing products. Identification. The photoelectrocolorimetric method for determining the mass concentration of polyphenols. Мoscow: Standartinform, 2021. 11 p. (In Russ.).
13. Ganeeva LА, Zaynulin LI, Abramova ZI, Tenisheva NH. Biochemistry. Workshop. Kazan: ISB, 2015. 176 p. (In Russ.).
14. Shi L, Zhao W, Yang Z, Subbiah V, Suleria HAR. Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environmental Science and Pollution Research. 2022;29(54):81112–81129. https://doi.org/10.1007/s11356-022-23337-6.
15. Chemat F, Rombaut N, Sicaire AG, Meullemiestre A, Fabiano-Tixier AS, Abert-Vian M. Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrason Sonochem. 2017;34:540–560. https://doi.org/10.1016/j.ultsonch.2016.06.035.
16. Almusallam IA, Ahmed IAM, Babiker EE, Al Juhaimi FY, Fadimu GJ, Osman MA, [et al.]. Optimization of ultrasound-assisted extraction of bioactive properties from date palm (Phoenix dactylifera L.) spikelets using response surface methodology. LWT. 2021;140:110816.
17. Sanz V, Torres MD, Lopez Vilarino JM, Dominguez H. Green extraction of phenolic compounds from Perle Hallertau and Nuggets hop pellets. Food Bioscience. 2022;50(A): 102044. https://doi.org/10.1016/j.fbio.2022.102044.
Authors
Kobelev Konstantin V., Doctor of Technical Science,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-3619-6282;
Gribkova Irina N., Candidate of Technical Science,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-4373-5387;
Borisenko Olga A.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-5281-444Х
All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow, 119021, Russia



Mukhamedzhanova T.G., Skorodumov A.S., Shanenko E.F., Moiseyak M.B., Nesterov E.D.Fermentation of Grape Pomace with E. cristatum to Obtain Extracts for the Production of Beverages

P. 9-13 DOI: 10.52653/PIN.2024.02.07

Key words
E. cristatum, grape pomace, waste disposal, secondary raw materials, extract, drink, post-fermentation

Abstract
The purpose of the study was to develop a method for producing a concentrated base for drinks based on grape marc using microbial fermentation with a culture of E. cristatum. For fermentation, a culture of the fungus Eurotium cristatum, anam. Aspergillus cristatus, No. F-1668, previously isolated from Fuzhuan tea was used. Methods for obtaining seed material of E. cristatum by deep cultivation using malt wort have been developed. The dynamics of the development of the fungus E. cristatum on grape pomace of the Cabernet Sauvignon variety was studied. The microbiological purity of the deep culture of the fungus used for inoculation of marc and finished fermented marc was determined. Organoleptic evaluation of fermented pomace showed that the extract is characterized by pronounced fruity and floral notes while maintaining the organoleptic properties of the original pomace.

References
1. Xiao Y, Huang Y, Chen Y, Fan Z, Chen R, He Ch, [et al.]. Effects of solid-state fermentation with Eurotium cristatum YL-1 on the nutritional value, total phenolics, isoflavones, antioxidant activity, and volatile organic compounds of black soybeans. Agronomy. 2021;11(6):1029. https://doi.org/10.3390/agronomy11061029.
2. Sharifi-Rad J, Bahukhandi A, Dhyani P, Sati P, Capanoglu E, Docea AO, [et al.]. Therapeutic potential of neoechinulins and their derivatives: an overview of the molecular mechanisms behind pharmacological activities. Frontiers in Nutrition. 2021;8:664197. https://doi.org/10.3389/fnut.2021.664197.
3. Mitra S, Anand U, Sanyal R, Jha NK, Behl T, Mundhra A, [et al.]. Neoechinulins: Molecular, cellular, and functional attributes as promising therapeutics against cancer and other human diseases. Biomedicine & Pharmacotherapy. 2022;145:112378. https://doi.org/10.1016/j.biopha.2021.112378.
4. Alhadrami HA, Burgio G, Thissera B, Orfali R, Jiffri SE, Yaseen M, [et al.]. Neoechinulin A as a promising SARS-CoV-2 Mpro inhibitor: In vitro and in silico study showing the ability of simulations in discerning active from inactive enzyme inhibitors. Marine Drugs. 2022;20(3):163. https://doi.org/10.3390/md20030163.
5. Xiao Y, Li M, Wu Y, Zhong K, Gao H. Structural characteristics and hypolipidemic activity of theabrownins from dark tea fermented by single species Eurotium cristatum PW-1. Biomolecules. 2020;10(2):204. https://doi.org/10.3390/biom10020204.
6. Xiao Y, Li M, Liu Y, Xu S, Zhong K, Wu Y, [et al.]. The effect of Eurotium cristatum (MF800948) fermentation on the quality of autumn green tea. Food Chemistry. 2021;358:129848. https://doi.org/10.1016/j.foodchem.2021.129848.
7. Lambert JD, Yang CS. Mechanisms of cancer prevention by tea constituents. The Journal of Nutrition. 2003;133 (10):3262S–3267S. https://doi.org/10.1093/jn/133.10.3262S.
8. Maruyama K, Ohuchi T, Yoshida K, Shibata Y, Sugawara F, Arai T. Protective properties of Neoechinulin A against SIN-1 — induced neuronal cell death. The Journal of biochemistry. 2004;136(1):81–87. https://doi.org/10.1093/jb/mvh103.
9. Nesterov ED, Loginov ZA, Kalinovskaya AI. Razrabotka sposoba polucheniya vinnogo napitka na osnove molochnoi syvorotki s ispol’zovaniem griba E. cristatum [Development of a method for producing a wine drink based on whey using E. cristatum mushroom]. Tendentsii razvitiya nauki i obrazovaniya= Trends in the development of science and education. 2022;(85-8):139–143. https://doi.org/10.18411/trnio-05-2022-376. (In Russ.).
10. Ivić I, Kopjar M, Jakobek L, Jukić V, Korbar S, Marić B, [et al.]. Influence of processing parameters on phenolic compounds and color of cabernet sauvignon red wine concentrates obtained by reverse osmosis and nanofiltration. Processes. 2021;9(1):89. https://doi.org/10.3390/pr9010089.
11. Lisov N, Petrovic A, Čakar U, Jadranin M, Tešević V, Bukarica-Gojković L, [et al.]. Extraction kinetic of some phenolic compounds during Cabernet Sauvignon alcoholic fermentation and antioxidant properties of derived wines. Macedonian Journal of Chemistry and Chemical Engineering. 2020;39(2):185–196. https://doi.org/10.20450/mjcce.2020.2060.
12. Yin J, Liu X, Peng F, Wang Q, Xiao Y, Liu S. Metabolite profiling, antioxidant and anti-aging activities of Siraitia grosvenorii pomace processed by solid-state fermentation with Eurotium cristatum. Process Biochemistry. 2023;133:109–120. https://doi.org/10.1016/j.procbio.2023.08.016.
13. Song J-L, Gao Y. Effects of methanolic extract form Fuzhuan brick-tea on hydrogen peroxide-induced oxidative stress in human intestinal epithelial adenocarcinoma Caco-2 cells. Molecular medicine reports. 2014;9(3):1061–1067. https://doi.org/10.3892/mmr.2014.1884.
14. Zakharov VL, Soldatova TA. Organoleptic and chemical indicators of types of tea from the fermented leaves of various plants of the lipetsk region. Mezhdunarodnyi zhurnal gumanitarnykh i estestvennykh nauk = International journal of humanities and natural sciences. 2016;(1-5):259–263. (In Russ.).
Authors
Muhamedzhanova Tat'yana G., Candidate of Technical Science, Аssociate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0003-1590-7601;
Shanenko Elena F., Candidate of Biological Science, Аssociate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0003-0875-9292;
Moyseyak Marina B., Candidate of Technical Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Russian Biotechnological University (ROSBIOTECH),
11, Volokolamskoe highway, Moscow, 125080, Russia
Skorodumov Aleksandr S.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-8905-255X;
Nesterov Egor D.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-3442-7024
LLC "Bavar+",
2, 2nd Mytishchinskaya Str., Moscow, 129626, Russia



Abramova I.M., Turshatov M.V., Kononenko V.V., Ledenev V.P., Soloviev A.O., Nikitenko V.D., Vakulovskaya N.A.Hardware and Technological Solutions for Complex Processing of Jerusalem Artichoke into Alcohol, Distillates and Dietary Fiber

P. 14-20 DOI: 10.52653/PIN.2024.02.06

Key words
Jerusalem artichoke, topinambur, Helianthus tuberosus, complex processing, distillate, alcohol, rectification, food processing, drying

Abstract
Jerusalem artichoke (lat. Helianthus tuberosus) is a promising crop for the production of a wide range of food and feed products. Currently, Jerusalem artichoke tubers are mainly used in the production of inulin, oligofructose syrups, fructose, pectin, and are also consumed raw. The green mass of Jerusalem artichoke ensiles well and is then used as a basis for creating feed products. However, existing technologies are not limited to obtaining only these products. Research carried out at VNIIPBT is aimed at the possibility of processing Jerusalem artichoke tubers and producing distillates with original organoleptic characteristics, alcohol and dietary fiber with different chemical compositions. To implement this technology on an industrial scale, it is necessary to analyze and develop hardware and technological solutions that will allow the implementation of a multi-product processing scheme. The paper examines the possibility of an integrated technology for processing Jerusalem artichoke tubers, producing distillates, alcohol and dietary fiber with maximum use of standard equipment of distilleries. The modes of water-heat treatment of the batch and an enzymatic complex containing xylanase, cellulase and beta-glucanase, which helps reduce the viscosity of the batch, have been determined. Experimental data were obtained on the possibility of dividing the medium into filtrate and dispersed fraction. In addition, the possibility of using the enzyme preparation polygalacturonase for the hydrolysis of pectin substances in raw materials was determined. Different types of drying devices for obtaining dietary fiber are considered. Studies have shown that the ratio of the volume of filtrate and wet sediment is 70 and 30%, respectively, while the content of dry substances in the filtrate is around 6,8±1,2%, and in dispersed sediment 12,4±0,6%. An algorithm for preparatory operations for processing Jerusalem artichoke for biotechnological processes, and a technological scheme for its direct processing, including drying, are presented.

References
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3. Tipsina, NN, Demidenko GA, Kokh DA. Technology of production and application of topinambour semi-finished products for bakery enrichment products. Polzunovskiy vestnik = Polzunovsky bulletin. 2023;(4):140–145. https://doi.org/10.25712/ASTU.2072-8921.2023.04.018. (In Russ.).
4. Turshatov MV, Abramova IM, Krivchenko VA, Solovyev AO, Nikitenko VD, Ledenev VP. Issledovanie fiziko-khimicheskikh svoistv topinambura s tsel’yu ego pererabotki v spirt i pishchevye funktsional’nye produkty [Investigation of physico-chemical properties of topinambur (the Jerusalem artichoke) with the purpose of its processing into alcohol and functional food products]. Mezhdunarodnyj kongress «Biotekhnologiya: sostoyanie i perspektivy razvitiya»: Materialy kongressa. Moscow, 2022. 197–199 p. (In Russ.).
5. Abramova IM, Turshatov MV, Krivchenko VA, Solovyev AO, Nikitenko VD. Issledovanie biokhimicheskogo sostava topinambura i poluchaemykh na ego osnove etilovogo spirta i pishchevykh funktsional’nykh produktov [Study of the biochemical composition of Jerusalem artichoke, as well as ethyl alcohol and food functional products obtained on its basis]. Biotekhnologiya = Biotechnology. 2022. P. 56–61. (In Russ.)
6. Abramova IM, Turshatov MV, Solovyev AO, Kononenko VV, Nikitenko VD. Research of topinambur water-heat and enzymatic processing modes for ethyl alcohol producing. IOP Conference Series Earth and Environmental Science. 2022;1112:012087. https://doi.org/10.1088/1755-1315/1112/1/012087.
7. Turshatov MV, Solovyev AO, Abramova IM, Kononenko VV, Nikitenko VD, Kryshchenko FI, [et al.]. Biotekhnologicheskie aspekty kompleksnoi pererabotki topinambura v etilovyi spirt i pishchevuyu kletchatku [Biotechnological aspects of the complex processing of article into ethyl alcohol and food fiber]. Teoreticheskie i prakticheskie aspekty razvitiya spirtovoi i likerovodochnoi promyshlennosti: sbornik nauchnykh trudov po materialam Mezhdunarodnogo nauchno-prakticheskogo seminara, Moscow, 2022. P. 101–108. (In Russ.).
8. Abramova IM, Solovyev AO, Kononenko VV, Pogorzhel’skaya NS, Yuraskina TV. Analiz variantov kompleksnoi pererabotki topinambura na spirt, distillyaty i pishchevuyu kletchatku [Analysis of options for the complex processing of Jerusalem artichoke for alcohol, distillates and dietary fiber]. Osnovnye napravleniya razvitiya tekhnologii glubokoi pererabotki krakhmalsoderzhashchego i inulinsoderzhashchego syr’ya: sbornik trudov mezhdunarodnoi nauchno-prakticheskoi konferentsii. Moscow, 2022. P. 8–14. (In Russ.).
9. Solovyev AO, Turshatov VV, Kononenko VV, Pogorzhel’skaya NS, Pavlenko SV. Issledovanie po pererabotke klubnei topinambura v distillyaty dlya proizvodstva original’nykh spirtnykh napitkov [Research on the processing of Jerusalem artichoke tubers into distillates for the production of original alcoholic beverages]. Industriya pitaniya = Food Industry. 2022;7(4):36–43. https://doi.org/10.29141/2500-1922-2022-7-4-4. (In Russ.).
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11. Kamilov KhCh., Ismoilova MA. Bezotkhodnaya pererabotka klubnei i zelenoi massy topinambura [Waste-free processing of tubers and green mass of Jerusalem artichoke]. Vestnik Tekhnologicheskogo universiteta Tadzhikistana = Bulletin of the Technological University of Tajikistan. 2019;(1(36)): 41–46. (In Russ.).
12. Shvets VI., Ivanova LA., Dvornik SL, Todosiichuk SR. Effektivnost’ razlichnykh sposobov podgotovki korneplodov topinambura k sbrazhivaniyu [The effectiveness of various methods of preparing jerusalem artichoke root crops for fermentation]. Izvestiya vysshih uchebnyh zavedenij. Pishchevaya tekhnologiya = Izvestiya vuzov. Food technology. 1995;(5–6);30–32. (In Russ.).
13. Turshatov MV, Solovyev AO, Volkova GS, Nikitenko VD. Kompleksnaya pererabotka topinambura s polucheniem pishchevykh funktsional’nykh produktov [Complex processing of Jerusalem artichoke to obtain functional food products]. Aktual’naya biotekhnologiya = Current biotechnology. 2022;(1):255. (In Russ.).
14. Bessonov VV, Bogachuk MN, Makarenko MA, Sokurenko MS, Shevyakova LV, Abramova IM, [et al.]. Study of the biochemical composition of grain fiber alcohol production. Pishchevaya promyshlennost’ = Food Industry. 2020;(2):12–15. https://doi.org/10.24411/0235-2486-2020-10014. (In Russ.).
15. Solovyev AO. The study of the rheological properties of the batch when obtaining alcohol from jerusalem artichoke. Pishchevaya promyshlennost’ = Food Industry. 2023;(5):39–41. https://doi.org/10.52653/PPI.2023.5.5.010. (In Russ.).
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17. Kirillov EA, Turshatov MV, Kononenko VV, Solov’ev AO, Alekseev VV. Modern research in the organization of the process of bragorectification in the production of alcohol from food raw materials. Pishchevaya promyshlennost’ = Food Industry. 2022;(8):54–56. https://doi.org/10.52653/PPI.2022.8.8.011. (In Russ.).
18. Konovalov VI, Kudra T, Koliukh AN, Romanova EV. Modern equipment for combined conductive convective drying and thermal processing. Vestnik Tambovskogo gosudarstvennogo tekhnicheskogo universiteta = Bulletin of the Tambov State Technical University. 2008;14(3):579–583. (In Russ.).
19. Tusipov NO. About driers for food products. Mezhdunarodnaya nauchno-prakticheskaya konferentsiya, posvyashchennaya pamyati Vasiliya Matveevicha Gorbatova = International scientific and practical conference dedicated to the memory of Vasily Matveevich Gorbatov. 2015;(1):460–462. (In Russ.).
Authors
Abramova Irina M., Doctor of Technical Science,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-9297-0554;
Turshatov Mikhail V., Candidate of Technical Science,
https://orcid.org/0000-0003-1277-5498;
Kononenko Valentin V., Candidate of Technical Science,
https://orcid.org/0000-0002-7568-5193;
Ledenev Vladimir P., Candidate of Technical Science,
https://orcid.org/ 0000-0003-3628-0289;
Solovyev Alexander O.,
https://orcid.org/0000-0003-2666-6890;
Nikitenko Viktoria D.,
https://orcid.org/0000-0001-5314-3513;
Vakulovskaya Natalia A.,
https://orcid.org/0009-0000-5639-0317
All-Russian Scientific Research Institute of Food Biotechnology - branch of the Federal State Budgetary Institution of Science Federal Research Center for Nutrition, Biotechnology and Food Safety,
4B, Samokatnaya Str., Moscow, 111033, Russia



TECHNOLOGY

Fataliyev H.K., Baloglanova K.V., Aghazade Y.E., Asgarova A.N., Heydarov E.E.Study of Some Factors Influencing the Production of High-quality Apple Material

P. 21-26 DOI: 10.52653/PIN.2024.02.10

Key words
juice, apple material, molds, infection, patulin, toxins

Abstract
In recent years, more than 50% of all fruit and berry production in Azerbaijan has been accounted for by apples, pomegranates, and persimmons. In the production of fruit, especially apples, inefficient utilization is not uncommon. The best fruits are selected and sent to retail chains for sale. Small apples, fruits that do not have a commercial appearance, or fruits damaged in any way are mostly discarded. One of the most convenient ways to eliminate such losses is to use these fruits in the production of long-lasting beverages, such as wine and calvados. Research has shown that apples damaged in one way or another primarily become the main nutrient substrate for mold fungi. Such fungus-infected fruit and the products derived from them have high levels of toxins produced by these fungi. The elimination of such drawbacks and the production of beverages of improved quality through the study of the above-described problems are relevant. The degree of contamination of fruits with toxins depending on the methods of damage and the types of microorganisms contained in them, as well as ways to eliminate this, were substantiated by experimental studies. A comprehensive technology has been developed to ensure the production of safe juices and wines for calvados by eliminating patulin, which is resistant to high temperatures, which also ensures the purification of raw materials from toxins. The addition of additives obtained from the press cake to the purified samples ensured the production of wines of improved quality. The research determined the optimal ratio of components, differing in chemical composition and quality, for the production of calvados. Thus, a blend of wine materials from Kyzyl Akhmedi (10%), Golden Delicious (30), Sary Tursh (10), Simirenko Reneti (20), Fuji (20), and Jyrgadzhi (10%) produces a blend material with high composite and organoleptic quality.

References
1. Mammadova SM, Fataliyev HK, Gadimova NS, Aliyeva GR, Tagıyev AT, Konul VB. Production of functional products using grape processing residuals. Food Science and Technology. 2020;40:422–428. https://doi.org/10.1590/fst. 30419.
2. Kamaladdin FH, Mecid MS, Mahir IM, Sefer QN, Telman IM, Elman HE, Musa MT. The study of resource saving technologies in the processing of grapes. Advances in Applied Science Research. 2020;11(3:2):1–5. https://doi.org/10.36648/0976-8610.11.3.2.
3. Baloglanova KV, Fataliyev HK, Alekberov AM. A study of the production of safe apple wort for calvados. Pivo i napitki = Beer and Beverages, 2020;(3):68–72. (In Russ.). https://doi.org/10.24411/2072-9650-2020-10023.
4. Fataliyev HK. Winemaking workshop. Baku: Science, 2013. 328 p. (In Az.).
5. Baloglanova KV, Tagiyev AT, Fataliyev KhK, Askerova AN. Study of phenolic compounds in apple juice // Vinodelie i vinogradarstvo = Winemaking and Viticulture. 2020;(2):19–23. (In Russ.).
6. Artık N, Velioglu S, Saglam N. Mikotoksinler: Patulin’in Oluşumu ve Meyve Sularındaki Önemi. Araştırma, 1991;3(33):13–17. (In Tr.).
7. Kadakal Ç, Nas S. Factors effecting to the amount of patulin in apple and apple products. Pamukkale University Journal of engineering sciences. 2000; 6(1):87–96. (In Tr.).
8. Starodub NF, Pilipenko LN, Egorova AV, Pilipenko IV. Mycotoxin patulin: producers, biological action, indication in food products. Sovremennye problemy toksikologii = Modern Problems of Toxicology. 2008;(3):50–57. (In Russ.).
9. Hopmans EC. Patulin: a Mycotoxinin Apples. Perishables Handing Quarterly. 1997; (91):5–6.
Authors
Fataliyev Hasil K., Doctor of Technical Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Aghazade Yahya E.,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Asgarova Aida N., Doctor of Technical Science, Associate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Azerbaijan state agricultural university,
262, Ataturk Avenue, Ganja, AZ2000 Republic of Azerbaijan
Baloglanova Konul V., Doctor of Technical Science,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Azerbaijan state oil and industry university,
20, Azadliq Avenue, Baku, AZ1010 Republic of Azerbaijan
Heydarov Elnur E., Doctor of Technical Science, Associate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Azerbaijan Technological University,
103 Shah Ismayil Khatai Avenue, Ganja, AZ2011 Republic of Azerbaijan



Moiseev I.V., Leznyy V.V., Karmanov D.A., Bondarenko D.V., Sinchin G.P.Enzymatic Changes in the Chemical Composition of Cigar Tobacco Raw Materials in an Alcohol-containing Medium of an Oak Barrel from Under Cognac Distillate

P. 27-30 DOI: 10.52653/PIN.2024.02.08

Key words
cigar tobacco, "alcoholic" fermentation, oak barrel, cognac distillate

Abstract
The influence of an alcohol-containing medium on the fermentation process of cigar tobacco raw materials in an oak barrel made from cognac distillate has been studied. A comparative assessment of two fermentation methods was carried out using the example of Cuban cigar tobacco of the HP variety. For this purpose, chemical analysis and tasting of unfermented tobacco and samples of similar tobacco that underwent natural and "alcoholic" fermentation for 6 months were carried out. Chemical analysis of the samples for main indicators was carried out using high-performance liquid chromatography, titrimetry and potentiometry methods. The results of chemical analysis on such indicators as the content of proteins, carbohydrates, nicotine, potassium and chlorine, as well as the hydrogen index revealed that in the alcohol-containing vapor-air environment of an oak barrel, oxidative processes occur more intensively compared with the "aging" of tobacco in an oak press. Of the other enzymatic processes, those that occur without the participation of water as a reagent are more intense. Also, the fermentation of tobacco in a gas-liquid alcohol-containing medium contributes to the "salting out" of mineral substances from raw tobacco. Tasting of the studied samples showed significant improvements in the flavor profile of tobacco smoke after fermentation of tobacco by both methods. However, fermentation in an alcohol-containing medium of cognac distillate has a clear advantage over natural fermentation. Due to the diffusion of aromatic substances from the alcohol distillate into tobacco, their diversity in the latter increases significantly, which makes it possible to create a special, unique flavor profile of the finished tobacco product. The results of these studies can be used by tobacco industry enterprises in the production of cigars, cigarillos, pipe tobacco and other niche tobacco products, as well as in the alcohol industry for the development and introduction to the consumer market of complementary structured products - tobacco products in a set with an alcoholic drink.

References
1. Leaves on a barrel or especially before aging tobacco [Internet]. [cited 2024 March 22]. URL: https://cigartime.ru/respectime/blogs/listya-na-bochku-ili-osobennosti-dovyderzhki-tabaka/ (In Russ.).
2. Kincurashvili KM. The process of «aging» of tobacco raw materials. Pivo I napitki = Beer and Beverages. 2006;(1):46–47. (In Russ.).
3. Moiseev IV, Karmanov DA, Leznyj VV. Quantitative changes of carboxylic acids in tobacco raw materials during fermentation. Pishchevaya promyshlennost’ = Food Industry. 2023;(11):18–21. https://doi.org/10.52653/PPI.2023.11.11.004. (In Russ.).
4. Moiseev IV, Karmanov DA, Leznyj VV, Kirillov DD. Quantitative change of nicotine in tobacco raw materials during natural fermentation under pressure. Nauchnyi zhurnal NIU ITMO. Seriya: Protsessy i apparaty pishchevykh proizvodstv = Processes and Food Production Equipment. 2022;(4(54)): 25–30. https://doi.org/10.17586/2310-1164-2022-15-4-25-30. (In Russ.).
5. Karmanov DA, Moiseev IV, Leznyy VV. Fermentation changes in the carbohydrate complex of tobacco. Agropromyshlennye tekhnologii Tsentral’noi Rossii = Agro-industrial technologies of Central Russia. 2024;1(31):36–42. https://doi.org/10.24888/2541-7835-2024-31-36-42. (In Russ.).
6. GOST R 54607.7–2016. Public catering services. Methods of laboratory quality control of products catering. Part 7. Determination of protein by the Kjeldahl method. Moscow: Standartinform, 2013. 12 p. (In Russ.).
7. Belyustin AA. Potentiometry: physico-chemical fundamentals and applications: Tutorial. Saint-Petersburg: Izdatel’stvo «Lan’», 2019. 336 р. (In Russ.).
8. Smirnova EYu, Gnuchih EV, Kirillov DD, Slavyanskij AA. Study of organoleptic and physical and chemical characteristics of products from smoking rolling tobacco. Novye tekhnologii = Technology of Food Production. 2022;18(3):85–93. https://doi.org/10.47370/2072-0920-2022-18-3-85-93. (In Russ.).
Authors
Moiseev Igor V., Doctor of Technical Science,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-9925-8850;
Leznyy Valerii V.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-1410-6936;
Karmanov Denis A.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-7317-4621;
Bondarenko Dmitriy V.,
This email address is being protected from spambots. You need JavaScript enabled to view it.
JSC "Pogar Cigarette & Cigar Factory",
41, Octyabrskaya Str., Pogar, Bryansk region, 243550, Russia
Sinchin Gregoriy P.,
This email address is being protected from spambots. You need JavaScript enabled to view it.
JSC "Derbent Brandy Factory",
56, Krasnoarmeysky Lane, Derbent, Republic of Dagestan, 368608, Russia



QUALITY CONTROL

Eliseev M.N., Tokarev P. I., Romashenko A.V.The Matcha Green Tea Quality Investigation

P. 31-35 DOI: 10.52653/PIN.2024.02.04

Key words
tea, matcha, organic compounds, quality, phenolic compounds, vitamins

Abstract
The article is devoted to the issue of researching matcha green tea. The goal of the work was to study tea samples to find identification indicators, which was solved using generally accepted methods of analysis. The authors showed that tea samples of Japanese origin had greater extract content compared to samples from China. Analysis of nitrogen fractions showed that matcha tea is more fermented because it contains more amino acids, regardless of the country of origin. The water-soluble fraction of soluble nitrogen is represented by amine nitrogen, and the glutelin fraction is 3-8 times lower than the content of the alkali-soluble fraction of other types of green tea. The total polyphenols content in matcha tea samples was in the range of 125.9-156.9 mg/g, catechins were higher than the control by 16% for sample No. 2 and 53% for sample No. 4. It has been shown that the tannin content in Chinese tea samples ranges from 2.18-3.29 mg%, in Japanese tea samples - 3.26-8.6 mg%. The lowest tannin content was observed in the Japanese matcha tea sample No. 1, the highest in tea No. 4, which depends on the processing conditions of the tea raw materials. In terms of caffeine content, the samples were in the range of 0.88-3.15%, which revealed a lack of correlation in sample No. 3, where the caffeine level was lower. The authors showed that the ratio of the amount of polyphenols and amino acids, the ratio of the amount of total polyphenols and nitrogen of the glutelin fraction can represent the identification parameters of matcha tea - a correlation was observed between these ratios and the content of catechins and tannins. It was shown that the chlorophyll content was in the range of 2.9-8.9 mg/g of tea, which is inversely correlated with the content of polyphenols and chlorophyll.

References
1. Horie H, Ema K, Sumikawa O. Chemical Components of Matcha and Powdered Green Tea. Journal of Cookery Science of Japan. 2017;50 (5):182–188. https://doi.org/10.11402/cookeryscience.50.182.
2. Kochman J, Jakubczyk K, Antoniewicz J, Mruk H, Janda K. Health Benefits and Chemical Composition of Matcha Green Tea: A Review. Molecules. 2021;26:85. https://dx.doi.org/10.3390/molecules26010085.
3. Koláčková T, Kolofiková K, Sytařová I. Matcha Tea: Analysis of Nutritional Composition, Phenolics and Antioxidant Activity. Plant Foods for Human Nutrition. 2020;75:48–53. https://doi.org/10.1007/s11130-019-00777-z.
4. Čížková H, Voldřich M, Mlejnecká J, Kvasnička F. Authenticity Evaluation of Tea-Based Products. Czech Journal of Food Sciences. 2008;26 (4):259–267. https://doi.org/10.17221/10/2008-CJFS.
5. Jakubczyk K, Kochman J, Kwiatkowska A, Kałdu´nska J, Dec K, Kawczuga D, Janda K. Antioxidant properties and nutritional composition of matcha green tea. Foods. 2020;9:483. https://doi.org/10.3390/foods9040483.
6. Кu KM, Choi JN, Kim J, Kim JK, Yoo LG, Lee SJ, [et al.]. Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia Sinensis L.). Journal of Agricultural and Food Chemistry. 2010;58:418–426. https://doi.org/10.1021/jf902929h.
7. Herrera M, Viera I, Roca M. HPLC-MS2 analysis of chlorophylls in green teas establishes differences among varieties. Molecules. 2022;27:6171. https://doi.org/10.3390/molecules27196171.
8. GOST R ISO 7513–2012. Instant tea. Determination of moisture content (loss in mass at 103 °C). Moscow: Standartinform, 2012. 9 p. (In Russ.).
9. GOST R ISO 9768–2011. Tea. Determination of water extract. Мoscow: Standartinform, 2011. 8 p. (In Russ.).
10. GOST ISO 10727–2013. Tea and instant tea. Determination of caffeine content. Method using high-performance liquid chromatography. Moscow: Standardinform, 2013. 13 p.
11. Maltcev PM, Velikaya EI, Zazirnaya MV, Kolotusha PV. Khimiko-tekhnologicheskiy kontrol’ proizvodstva soloda i piva [Chemical-technological control of malt and beer production]. Moscow: Pischevaya promishlenost publ., 1976. 448 p. (In Russ.).
12. GOST 34789–2021. Brewing Products. IDENTIFICATION. Determination of mass concentration of total nitrogen by Kjeldahl method. Moscow: Standartinform, 2021. 21 p. (In Russ.).
13. GOST R 55488–2013. Propolis. Method for the determination of polyphenols. Мoscow: Standartinform, 2013. 9 p. (In Russ.).
14. GOST 19885–74. Tea. Methods for determination of tannin and caffeine content. Мoscow: Standartinform, 2015. 5 p. (In Russ.).
15. Maslyanikov PV, Chupahina GN, Skryipnik LN, Fedurayev PV, Seledtcov VI. Ekologicheskiy analiz aktivnosti nakopleniya bioflavanoidov v lekarstvennykh rasteniyakh [Ecological analysis of the activity of accumulation of bioflavonoids in medicinal plants]. Vestnik Baltiyskogo federal’nogo universiteta im. K.I. Kanta = Bulletin of the Baltic Federal University named after K.I. Kant. 2014;(7):110–120. (In Russ.).
16. Naymushina LV, Karaseva AYu, Chesnokova NV. Spektrofotometricheskoye issledovaniye nakopleniya khlorofilla i yego proizvodnykh v ekstraktakh melissy lekarstvennoy pri ispol’zovanii dvukhfaznoy sistemy rastvoriteley [Spectrophotometric study of the accumulation of chlorophyll and its derivatives in lemon balm extracts using a two-phase solvent system]. Journal of Siberian Federal University. Chemistry. 2012;3(5):281–288. (In Russ.).
17. Adnan M, Ahmad A, Ahmed DA, Khalid N, Hayat I, Ahmed I. Chemical composition and sensory evaluation of tea (Camellia sinensis) commercialized in Pakistan. Pakistan Journal of Botany. 2013;45:901–907.
18. Zhang Y, Chen H, Zhang N, Ma L. Antioxidant and functional properties of tea protein as affected by the different tea processing methods. Journal of Food Science and Technology. 2015;52(2):742–752. https://doi.org/10.1007/s13197-013-1094-8.
19. Lin Z, Wei H, Zhang Y, Liu P, Liu Y, Huang Z, [et al.]. Improving emulsification properties of alkaline protein extract from green tea residue by enzymatic methods. Current Research in Food Science. 2022;5:1235–1242. https://doi.org/10.1016/j.crfs.2022.07.016.
20. Kaneko S, Kumazawa K, Masuda H, Henze A, Hofmann T. Molecular and sensory studies on the umami taste of Japanese green tea. Journal of Agricultural and Food Chemistry. 2006;54:2688–2694. https://doi.org/10.1021/jf0525232.
21. Nishitani E, Sagesaka YM. Simultaneous Determination of catechins, caffeine and other phenolic compounds in tea using new HPLC method. Journal of Food Composition and Analysis. 2004;17:675–685. https://doi.org/10.1016/j.jfca.2003.09.009.
22. Afonina SN, Lebedeva EN, Setko NP. Biochemistry of the components of tea and especially its biological effect on the organism (review). Orenburgskiy meditsinskiy vestnik = Orenburg Medical Bulletin. 2017;V(4):17–33. (In Russ.).
23. Luo Y, Zhang Y, Qu F, Qian W, Wang P, Zhang X, [et al.]. Variations of main quality components of matcha from different regions in the Chinese market. Frontiers in Nutrition. 2023;10:1153983. https://doi.org/10.3389/fnut.2023.
24. Suzuki Y, Shioi Y. Identification of chlorophylls and carotenoids in major teas by High-Performance Liquid Chromatography with Photodiode Array Detection. Journal of Agricultural and Food Chemistry. 2003;51:5307–5314. https://doi.org/10.1021/jf030158d.
Authors
Eliseev Michail N., Doctor of Technical Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-8636-4468;
Tokarev Petr I., Doctor of Biological Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Romasсhenko Anna V.,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Russian University of Economics named after G.V. Plekhanov,
36, Stremyanny lane, Moscow, 117997, Russia



Kaluzhina O.Yu., Lukin A.A., Tikhonenko M.A., Nafikova A.R.Comprehensive Research and Identification of Microplastics in Low-alcohol Drinks

P. 36-41 DOI: 10.52653/PIN.2024.02.05

Key words
beer, microplastic identification, low-alcohol drinks, microplastic contamination

Abstract
Plastic products have long made our lives easier. They are used in almost all industries. Due to its versatility and availability, plastic is considered one of the best materials for food preservation. However, under the influence of external forces, deformation, exposure to UV and other factors, plastic can disintegrate into small fragments, which are called microplastics. Currently, due to the difficulty of detecting the presence of microplastics, they can be classified as emerging pollutants. It has been proven that the entry of microplastics into biotic organisms can cause physical and oxidative stress, necrosis, apoptosis, inflammation and other immune reactions. This study focused on identifying ways in which low-alcohol drinks are contaminated with microplastics. Six samples of beer bottled in plastic bottles from six different brands were examined. The bottles were made of PET. The identification of microplastics in beer was carried out using FTIR (Fourier transform infrared) imaging spectrometry, which is a widely used method for detecting and identifying microplastics in complex matrices such as food and beverages. Analysis of beer samples showed the presence of microplastics in all six samples. Microplastic concentrations ranged from 113 particles/500 ml to 244 particles/500 ml. The most common microplastics were CE (51% of total), PET (20% of total) and PA (20% of total). Other types of microplastics were also found, including PP, PVC and PS. In terms of size, most of the identified microplastics were in the range of 50-100 and 100-300 µm. Fibers were the most common type of microplastic particles detected, accounting for 68.2% of the total microplastic particles. Possible sources of contamination include raw materials, ambient air, and equipment and packaging that release microplastics.

References
1. Isrigova TA, Lukin AA. Contamination of food and agricultural products with microplastics: a literature review. Izvestiya Dagestanskogo GAU = Dagestan GAU Proceeding. 2023;(1):173–178. (In Russ.). https://doi.org/10.52671/26867591_2023_1_173.
2. Oßmann BE, Sarau G, Holtmannspötter H, Pischetsrieder M, Christiansen SH, Dicke W. Small-sized microplastics and pigmented particles in bottled mineral water. Water Research. 2018;141:307–316. https://doi.org/10.1016/j.watres.2018.05.027.
3. Smith MM, Love CM, Rochman RA, Neff RA. Microplastics in seafood and the implications for human health. Current Environmental Health Reports. 2018;5:375–386. https://doi.org/10.1007/s40572-018-0206-z.
4. Liebezeit G, Liebezeit E. Non-pollen particulates in honey and sugar. Food Additives & Contaminants: Part A. 2013;30(12):2136–2140. https://doi.org/10.1080/19440049.2013.843025.
5. Dolder S, Hillman A, Passinsky V, Wooster K. Strategic analysis of water use in the beverage industry master thesis [Internet]. [cited 2023 December 5]. URL: https://deepblue.lib.umich.edu/bitstream/handle/2027.42/90925/MP_WaterRiskReport_Final_Delivered.pdf.
6. Kutralam-Muniasamy G, Pèrez-Guevara F, Elizalde-Martìnez I, Shruti VC. Branded milks — are they immune from microplastics contamination? Science of The Total Environment. 2020;714:136823. https://doi.org/10.1016/j.scitotenv.2020.136823.
7. Analysis of the low-alcohol drinks market in Russia. [Internet]. [cited 2023 December 5]. URL: https://businesstat.ru/russia/food/alcohol/low-alcohol_beverages/?yclid=9084497635583459327. (In Russ.).
8. Liebezeit G, Liebezeit E. Synthetic particles as contaminations in German beers. Food Additives & Contaminants: Part A. 2014;31(9):1574–1578. https://doi.org/10.1080/19440049.2014.945099.
9. Shruti VC, Perez-Guevara F, Elizalde-Martinez I, Kutralam-Muniasamy G. Toward a unified framework for investigating micro (nano) plastics in packaged beverages intended for human consumption. Environmental Pollution. 2021;268(A): 115811. https://doi.org/10.1016/j.envpol.2020.115811.
10. Cox KD, Covernton GA, Davies HL, Dower JF, Juanes F, Dudas SE. Human consumption of microplastics. Environmental Science & Technology. 2019;53(12):7068–7074. https://doi.org/10.1021/acs.est.9b01517.
11. Prata JC, Paço A, Reis V, da Costa JP, Fernandes AJS, da Costa FM, et al. Identification of microplastics in white wines capped with polyethylene stoppers using micro-Raman spectroscopy. Food Chemistry. 2020;331:127323. https://doi.org/10.1016/j.foodchem.2020.127323.
12. Zhou XJ, Wang J, Ren JF. Analysis of Microplastics in Takeaway Food Containers in China Using FPA-FTIR Whole Filter Analysis. Molecules. 2022;27(9):2646. https://doi.org/10.3390/molecules27092646.
13. Gupta, S., Kumar, R., Rajput, A., [et al.]. (2023). Atmospheric Microplastics: Perspectives on Origin, Abundances, Ecological and Health Risks. Environmental Science and Pollution Research. 2023;30:107435–107464. https://doi.org/10.1007/s11356-023-28422-y.
14. Dris R, Gasperi J, Saad M, Mirande C, Tassin B. Synthetic fibers in atmospheric fallout: A source of microplastics in the environment? Marine Pollution Bulletin. 2016;104(1–2):290–293. https://doi.org/10.1016/j.marpolbul.2016.01.006.
15. Dessì C, Okoffo ED, O’Brien JW, Gallen M, Samanipour S, Kaserzon S, Rauert C, Wang XY, Thomas KV. Plastics contamination of store-bought rice. Journal of Hazardous Materials. 2021;416:125778. https://doi.org/10.1016/j.jhazmat.2021.125778.
16. Liu YY, Guo R, Zhang SW, Sun YH, Wang FY. Uptake and translocation of nano/microplastics by rice seedlings: Evidence from a hydroponic experiment. Journal of Hazardous Materials. 2022;421:126700. https://doi.org/10.1016/j.jhazmat.2021.126700.
Authors
Kaluzhina Olesya Yu., Candidate of Technical Science, Аssociate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-5923-1231;
Nafikova Aigul R.,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0002-0741-6354
Bashkir State Agrarian University,
34, 50-letia Octyabrya Str., Ufa, 450001, Russia
Lukin Alexander A., Candidate of Technical Science, Аssociate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/ 0000-0003-4753-3210
South Ural State University,
76, Lenin Avenue, Chelyabinsk, 454080, Russia
Tikhonenko Marina A.,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Agrofirma Ariant LLC,
211, Blucher Str., Chelyabinsk, 454901, Russia



RAW and OTHER MATERIALS

Tokarev P. I., Eliseev M.N., Red'ko I.V.The SCOBY Drinks Quality Investigation

P. 42-45 DOI: 10.52653/PIN.2024.02.03

Key words
fermented drinks, raw materials, SCOBY, organic compounds, vitamins

Abstract
The article is devoted to the fermented drinks quality investigation which using a consortium of microorganism's SCOBY, produced industrially. It is noted that drinks in an accessible form contain important organic compounds (organic acids, phenolic compounds, minerals, vitamins, etc.), considering the minimum amount of ethanol in the composition. This fact is very relevant from the point of view of its positive effect on the body, so it is important to study the quality of this type of drink, which is the purpose of this article. The study was carried out on the basis of standard analytical methods, including instrumental ones, accepted in the industry. It was shown that the alcohol content in the samples complies with the requirements of 171-Federal Law and does not exceed 1.2 vol.%; according to the level of the actual extract, all samples are grouped into group 1 (1.1-1.2%) and group 2 (6.5-7.2%). The acidity level in the first two samples is close, samples 3 and 4 were 13 and 78% higher than the acidity level of group 1 samples. The color of the drinks correlated with the level of polyphenols, including catechins, and the content of the actual extract. The content of polyphenols in the first drinks group was in the range of 123.0-147.6 mg/dm3, and in the second group it exceeded the values of group 1 by 4-6 times. Catechins were correlated in content with total polyphenols; in the first group of drinks they were contained at a level of 4.45-4.95 mg/dm3, and in the second - 6.19-6.48 mg/dm3, which in turn correlates with the type of drink used in raw materials technologies. It was shown that lactic and acetic acids were predominant in quantity in drinks. Thus, the range of lactic acid content ranged from 0.27-1.67 g/dm3, and acetic acid - 1.83-3.83 g/dm3. The highest total content of organic acids was observed in drink sample No. 4, the lowest - in No. 3. It was noted that there is no correlation between the amounts of different organic acids. It was found that the amount of vitamin B2 (riboflavin) depended on the list of raw materials used and correlated with the total content of organic compounds.

References
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4. GOST 12787–2021. Brewing products. Methods for determining the volume fraction of ethyl alcohol, the mass fraction of the actual extract and calculating the extract content of the initial wort. Мoscow: Standsrtinform, 2021. 21 p. (In Russ.).
5. GOST 12788–87. Beer. Methods for determining acidity. Мoscow: Standartinform, 2011. 4 p. (In Russ.).
6. GOST 12789–2022. Brewing products. Color determination methods. Мoscow: Standartinform, 2022. 9 p. (In Russ.).
7. GOST 34798–2021. Brewing products. Identification. Photoelectrocolorimetric method for determining the mass concentration of polyphenols. Мoscow: Standartinform, 2021. 12 p. (In Russ.).
8. Maslyannikov PV, Chupahina GN, Skryipnik LN, Fedurayev PV, Seledtcov VI. Ekologicheskiy analiz aktivnosti nakopleniya bioflavanoidov v lekarstvennykh rasteniyakh [Ecological analysis of bioflavonoids accumulation activity in medicinal plants]. Vestnik Baltiiskogo federal’nogo universiteta im. K.I. Kanta = Kant’s Bulletin Baltic federal university. 2014;(7):110–120. (In Russ.).
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12. Shanenko EF, Skorodumov AS, Mukhamedzhanova TG, Barkanov AV, Grushnikova VI, Manin ES, [et al.]. Prospects for the use of SCOBY in the technology of non-alcoholic fermented beverages. Pivo i napitki = Beer and beverages. 2024;(1):10–17. (In Russ.). https://doi.org/10.52653/PIN.2024.01.06.
13. Samoylenko МV, Babakina МV, Zolotavina МL. Opredeleniye biokhimicheskikh priznakov mikrobiologicheskikh kul’tur, vkhodyashchikh v sostav konsortsiuma SCOBY [Determination of biochemical characteristics of microbiological cultures that are part of the SCOBY consortium]. Mezhdunarodnyi zhurnal gumanitarnykh i estestvennykh nauk = International Journal of humanities and natural sciences. 2021;11-3(62):11–14. (In Russ.). https://doi.org/10.24412/2500-1000-2021-11-3-11-14.
14. Afonina SN, Lebedeva EN. Khimicheskiye komponenty chaya i ikh vliyaniye na organizm [Chemical components of tea and their effect on the body]. Uspekhi sovremennogo estestvoznaniya = Advances in modern natural science. 2016;(6):59–63. (In Russ.).
15. Kruk M, Trząskowska M, Ścibisz I, Pokorski P. Application of the «SCOBY» and kombucha tea for the production of fermented milk drinks. Microorganisms. 2021;9(1):123. https://doi.org/10.3390/microorganisms9010123.
16. Flyurik EA, Ermakova OS. Medusomyces gisevii L.: cultivation, composition, and application. Foods and Raw Materials. 2023;11(1):152–161. http://doi.org/10.21603/2308-4057-2023-1-563.
Authors
Tokarev Petr I., Doctor of Biological Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Eliseev Michail N., Doctor of Technical Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. , https://orcid.org/0000-0001-8636-4468;
Red'ko Irina V.,
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Russian University of Economics named after G.V. Plekhanov,
36, Stremyanny lane, Moscow, 117997, Russia



Karpenko D.V., Matveev S.V., Morenkov N.V., Morozov D.A., Perevezenkov P.S.Enzyme Preparations in Malt Production and Brewing: a Range of Tasks to be Solved. Part III

P. 46-51 DOI: 10.52653/PIN.2024.02.01

Key words
brewing, enzyme preparations, intensification of main fermentation and post-fermentation, acceleration of beer filtration, increasing colloidal stability and quality of beer

Abstract
The article systematizes and analyzes information about enzyme preparations of various origins and types of action, proposed for use to intensify technological processes at the stages of malting and brewing production. The advantages provided by the rational use of enzyme preparations are presented. It is noted that one of these advantages is an increase in the degree of attenuation of the wort due to the additional saccharification of medium-molecular starch hydrolysis products remaining in the wort after mashing and filtering of the mash, due to the action of amylases of the introduced preparation, and in dosages significantly lower than at earlier stages technological process. Another important characteristic that can be improved with the help of extraneous preparations is the viscosity of beer, the reduction of which intensifies the filtering process. When producing a drink using gravity and high-gravity brewing technology, a decrease in the concentration in the nutrient medium of low-molecular nitrogen compounds digestible by yeast is inevitable. To eliminate this problem, it is recommended to use exogenous proteases at the stage of main fermentation, under the influence of which the concentration of compounds of this group increases, which leads to an increase in the degree of attenuation, ethanol yield, and improvement in the organoleptic characteristics of the finished beer. Another important goal is to accelerate the reduction of diacetyl concentration under the action of ?-acetolactate decarboxylase, which allows reducing the duration of the post-fermentation and maturation stage of brewing. The use of enzyme preparations ensures the production of beer with higher initial clarity and longer colloidal stability. In conclusion, it is noted that all these results can be achieved through the use of enzyme preparations with the appropriate specificity of the target enzymes, however, most of the used auxiliary products of this group are produced or supplied by Western companies, which currently reduces their availability for domestic enterprises. The opinion was expressed that it would be advisable to study the possibility and effectiveness of using similar enzyme preparations produced in Southeast Asia, information about which is still insufficient in the scientific literature.

References
1. Karpenko DV, Matveev SV, Morenkov NV, Morozov DA, Runjova DA. Enzyme preparations in malt production and brewing: a range of tasks to be solved. Part II. Pivo i napitki = Beer and beverages. 2024;(1):52–64. https://doi.org/10.52653/PIN.2024.01.01.
2. Meledina TV. Syr’e i vspomogatel’nye materialy v pivovarenii [Raw materials and auxiliary materials in brewing]. Saint-Petersburg: Profession, 2003. 318 p. (In Russ.).
3. Zymus Brewing enzymes and auxiliaries [Internet]. [cited 2023 September 2]. URL: https://zymus.net/products/brewing-enzymes-and-auxiliaries/.
4. Fermentnye preparaty dlja primenenija v pivovarenii [Enzyme preparations for use in brewing]. [Internet]. [cited 2023 September 2]. URL: https://www.calameo.com/read/0055096149f383196a67d. (In Russ.).
5. Karpenko DV. Kaledin IM. Increasing the colloidal stability of non-alcoholic beer using «BREWERS CLAREX» enzyme preparation. Health, Food & Biotechnology. 2022;4(2):68–77. https://doi.org/10.36107/hfb.2022.i2.s142. (In Russ.).
6. Ul’traflo kore β-gljukanaza dlja uluchshenija reologii pivnogo susla [Ultraflo core β-glucanase to improve the rheology of beer wort] [Internet]. [cited 2023 August 25]. URL: https://rusferment.com/fermenty/ultraflo-kore. (In Russ.).
7. Gomaa A. Application of enzymes in brewing. Journal of Nutrition and Food Science Forecast. 2018;1(1):1002. https://doi.org/10.5281/zenodo.3336203.
8. Carbonell JV, Sendra JM, Todo V. Kinetics of β-glucan degradation in beer by exogenous β-glucanase treatment. Journal of the Institute of Brewing. 1990;96(2):81–84.
9. Piddocke MP, Fazio A, Vongsangnak W, Wong ML, Heldt-Hansen HP, Workman C, [et al.]. Revealing the beneficial effect of protease supplementation to high gravity beer fermentations using «-omics» techniques. Microbial Cell Factories. 2011;10:27. https://doi.org/10.1186/1475-2859-10-27.
10. Lei H, Zhao H, Zhao M. Proteases supplementation to high gravity worts enhances fermentation performance of brewer’s yeast. Biochemical Engineering Journal. 2013;77:1–6. https://doi.org/10.1016/j.bej.2013.04.016.
11. Krogerus K, Gibson B. 125th Anniversary Review: Diacetyl and its control during brewery fermentation. Journal of the Institute of Brewing. 2013;119(3):86–97. https://doi.org/10.1002/jib.84.
12. Dulieu C, Moll M, Boudrant J, Poncelet D. Improved performances and control of beer fermentation using encapsulated alpha-acetolactate decarboxylase and modeling. Biotechnology progress. 2000;16(6):958–965. https://doi.org/10.1021/bp000128k.
13. Kharandiuk TV, Kosiv RB, Borukh OM, Dalybozhyk RS, Palianytsia LYa, Berezovska NI. Decreasing of vicinal diketones content during fermentation of high gravity wort. L’vovskii natsional’nyi universitet veterinarnoi meditsiny i biotekhnologii imeni S.Z. Gzhitskogo = Scientific messenger LNUVMBT named after S.Z. Gzhytskyj. 2017;19(5):149–152. (In Ukr.) https://doi.org/10.15421/nvlvet7530.
14. Fermenty dlja piva i kvasa. Al’falaza Advance 4000 [Enzymes for beer and kvass. Alphalase Advance 4000] [Internet]. [cited 2023 September 3]. URL: https://xn--80adfdatufkwf1au8huc.xn--p1ai/katalog-fermentov/alphalase-advance-4000/ (In Russ.).
15. Karmanova LV. Application of collagenase enzyme preparation in brewing. Beer and beverages. 2000;(5):44–45. (In Russ.).
16. Lopez M, Edens L. Effective prevention of chill-haze in beer using an acid proline-specific endoprotease from Aspergillus niger. Journal of agricultural and food chemistry. 2005;53(20):7944–7949. https://doi.org/10.1021/jf0506535.
17. Ogata T, Iwashita Y, Kawada T. Construction of a brewing yeast expressing the glucoamylase gene STA1 by mating. Journal of the Institute of Brewing. 2017;123(1):66–69. https://doi.org/10.1002/jib.394.
18. Brewlyve for brewing & distilling [Internet]. [cited 2023 August 20]. URL: https://www.souffletbiotechnologies.com/wp-content/uploads/2022/12/Fiche-A4-Brewlyve.pdf.
19. Tolkacheva AA, Cherenkov DA, Korneeva OS, Ponomarev PG. Enzymes of industrial purpose — review of the market of enzyme preparations and prospects for its development. Proceedings of the Voronezh State University of Engineering Technologies. 2017;79(4):197–203. https://doi.org/10.20914/2310-1202-2017-4-197-203. (In Russ.).
Authors
Karpenko Dmitry V., Doctor of Technical Science, Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Matveev Sergej V.,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Morenkov Nikolaj V.,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Morozov Dmitry A.,
This email address is being protected from spambots. You need JavaScript enabled to view it. ;
Perevezenkov Pavel S.,
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Russian Biotechnological University (ROSBIOTECH),
11, Volokolamskoye highway, Moscow, 125080, Russia



Khokonova M.B.Comparative Assessment of Brewing Qualities of Winter and Spring Barley Varieties

P. 52-54 DOI: 10.52653/PIN.2024.02.09

Key words
barley, varieties, crop structure, yield, brewing qualities

Abstract
The possibility of using winter and spring barley grown in the Kabardino-Balkarian Republic for the brewing industry was investigated under production conditions. The research objects were winter barley varieties - Master, Mikhailo, Kozyr, Dobrynya 3 and spring barley - Priazovsky 9, Getman, Viscount, Mamluk, approved for use in the North Caucasus region. The Master variety has the greatest winter hardiness. Mikhailo showed a figure close to his. Kozyr and Dobrynya 3 were characterized by reduced winter hardiness, by 12-14% compared to the first variety. The highest indicators for them were noted in the Priazovsky 9 variety - 45.2 g with 21 grains per year. Mamluk differs from the listed varieties in the smaller grain size of the ear - 19 pieces. and the minimum among them with an absolute grain weight of 41.6 g. A high coefficient of productive tillering was noted in the Master variety, due to which there were 3,42 million ears per 1 hectare with the largest number of grains per ear - 32 pieces. - and their absolute weight - 42.9 g. The remaining varieties were inferior to it in the number of plants per 1 m2 by 3.4-13.3%, the number of productive stems by 4.4-17.8%, the number of grains per year by 3.3-31.2%, absolute grain weight by 1.2-4.0%. The spring varieties Viscount and Mamluk had an average grain size, while in the Priazovsky 9 and Getman varieties it was 3-4% higher. Of the proposed winter varieties, the Master variety distinguished itself by the largest size. It also had the maximum indicators in terms of nature and weight of 1000 grains, percentage of starch and extract content. Mikhailo and Kozyr have similar values for all of the above indicators and are inferior to the Master in grain size by 13.0-17.2%, weight of 1000 grains by 0.9-1.9%, natural weight - by 2.5-3.4%, starch content - by 9.1-10.0%, extract content - by 3.2-1.6%.

References
1. Goncharov SV, Fedotov VA, Matveev IV, Tarakanovskii AN, Kalakutskii KL, Potemkin VA, [et al.]. Pivovarennyi yachmen’ [Malting barley]. Moscow: Singenta, 2014. 288 p. (In Russ.).
2. Kurochkin AA., Voronina PK., Shaburova GV. Teoreticheskoe obosnovanie primeneniya ekstrudirovannogo syr’ya v tekhnologiyakh pishchevykh produktov [Theoretical justification for the use of extruded raw materials in food technologies]. Penza. IP Popova M.G., 2015. 182 p. (In Russ.).
3. Shaburova GV., Kurochkin AA., Voronina PK. Increasing the technological potential of unmalted grain products. Tehnika i tehnologija pishhevyh proizvodstv = Equipment and technology of food production. 2014;(1):90–96. (In Russ.).
4. Hokonova MB. Application of enzyme preparations in the production of brewing malt. Izvestija Kabardino-Balkarskogo GAU = News of the Kabardino-Balkarian State Agrarian University. 2016;(1):50–54. (In Russ.).
5. Hokonova MB. The quality of malting barley depending on the slope of the growing area. Pivo i napitki = Beer and drinks. 2015;(3):48–51. (In Russ.).
6. Khokonova MB., Adzhieva AA. Photosynthetic activity of spring barley plants depending on moisture provision. Amazonia Investiga. 2019;8(23):96–100.
Authors
Khokonova Madina B., Doctor of Agricultural Science, Associate Professor,
This email address is being protected from spambots. You need JavaScript enabled to view it.
Kabardino-Balkarian State Agrarian University named after V.M. Kokov,
1V, Lenin avenue, Nalchik, Kabardino-Balkarian Republic, 360030, Russia



INFORMATION

International Exhibition of Equipment, Ingredients and Packaging for Beverage Production BeviTec 2024

Ermolaeva G.A. The Biomass Forum: Fuel and Energy - 2024 was held in Moscow