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Beer and beverages №4/2023


Zakharov M.A., Zakharova V.A., Gribkova I.N.The Hop Nutraceutical's Transfer from Processed Brewer's Spent Grain Structure and Their Qualitative Composition

P. 4-9 DOI: 10.52653/PIN.2023.04.003

Key words
brewer's spent grain, physical processing, ECA-water, grain structure, nutraceutical compounds

The article is devoted to the possibility of transporting hop extract's organic compounds by the processed brewer's spent grain structures issue. The work purpose was to study the environmental processing methods influence on changes in the structure of the brewer's spent grain ligninocellulosic complex to extract compounds with proven pharmacological properties using hop extracts as an example. The goal set by the authors - to study the influence of processing brewer's spent grain environmental methods on the possibility of sorption-desorption of hop's nutritional compounds - was solved using instrumental analysis methods (GC-MS). It was shown that the compounds identified by the authors that are transported by spent grain depend on both the hop's type and the brewer's spent grain processing method. Methylated propanoic acid is sorbed/desorbed only from Magnum hop variety extract by both forms of processed brewer's spent grain, and the transfer of its derivatives (ether and chlorine derivatives) is more typical for Tettnanger hop variety. The presence of butanoic acid and the it's ester methyl derivative is characteristic of Magnum hop variety extract, while the ester of this carboxylic acid is found in Tettnanger hop variety extract. There was identified 2-methyl-,1,3-bis(acetyloxy)-3,5,6,6a,7,8,9,10-octahydro-9,10-dihydroxy-7,8-dimethyl-7-(3-methylene-4-penten-1-yl)-1H-naphtho[1,8a-c]furan-5-yl ester of butanoic acid, sesquiterpene lactone kalein, alkaloid substance N-methylasimilobin N-oxide, ilicolinum, hydrocortisone acetate (corticosteroid), cyclopenthiazide (hyposthenic), chlordiazepoxide, trimethoprim in the samples. Their presence was identified in all cases. Other compounds [5,5-dimethyl- (5H)-2-furanone; 3,7,11,15-tetramethyl-2-hexadecen-1-ol; 4-methyl-2-pentyl-1,3-dioxolane, isoleden] were not identified in all cases. It was shown that the processes of transfer of nutraceutical compounds were ultimately influenced by three factors: the structure of the compounds (hop type), the method of processing the grains (isobaric or ultrasonic treatment), and the type of extractant.

1. Kavalopoulos M, Stoumpou V, Christofi A, Mai S, Barampouti EM, Moustakas K, Malamis D, Loizidou M. Sustainable valorisation pathways mitigating environmental pollution from brewers' spent grains. Environmental Pollution. 2021;270:116069. https://doi.org/10.1016/j.envpol.2020.116069.
2. Mitri S, Salameh S-J, Khelfa A, Leonard E, Maroun RG, Louka N, Koubaa M. Valorization of Brewers' Spent Grains: Pretreatments and Fermentation, a Review. Fermentation. 2022;8:50. https://doi.org/10.3390/fermentation8020050.
3. Forssell P, Kontkanen H, Schols H, Hinz S, Vincent G, Buchert J. Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes. Journal of the Institute of Brewing. 2008;114 (4):306-314. https://doi.org/10.1002/j.2050-0416.2008.tb00774.x.
4. Cooray ST, Chen WN. Valorization of brewer's spent grain using fungi solid-state fermentation to enhance nutritional value. Journal of Functional Foods. 2018;42:85-94. https://doi.org/10.1016/j.jff.2017.12.027.
5. Slawomir W, Klos A. Heavy metal sorption in biosorbents - Using spent grain from the brewing industry. Journal of Cleaner Production. 2019;225:112-120. https://doi.org/10.1016/j.jclepro.2019.03.286.
6. Carrasco KH, Hofgen EG, Brunner D, Borchert KBL, Reis B, Steinbach C, [et al.]. Removal of iron, manganese, cadmium, and nickel ions using brewers' spent grain. Polysaccharides. 2022;3:356-379. https://doi.org/10.3390/polysaccharides3020021.
7. Rutnik K, Ocvirk M, Kosir IJ. Changes in Hop (Humulus lupulus L.) oil content and composition during long-term storage under different conditions. Foods. 2022;11(19):3089. https://doi.org/10.3390/foods11193089.
8. Sleha R, Radochova V, Malis J, Mikyska A, Houska M, Krofta K, [et al.]. Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected ExternalWounds In Vivo. Antibiotics. 2021;10(6):708. https://doi.org/10.3390/antibiotics10060708.
9. Bogdanova K, Roderova M, Kolar M, Langova K, Dusek M, Jost P, [et al.]. Antibiofilm activity of bioactive hop compounds humulone, lupulone and xanthohumol toward susceptible and resistant staphylococci. Research in Microbiology. 2018;169(3):127-134. https://doi.org/10.1016/j.resmic.2017.12.005.
10. Cermak P, Olsovska J, Mikyska A, Dusek M, Kadleckova Z, Vanicek J, [et al.]. Strong antimicrobial activity of xanthohumol and other derivatives from hops (Humulus lupulus L.) on gut anaerobic bacteria. APMIS. 2017;125(11):1033-1038. https://doi.org/10.1111/apm.12747.
11. Roehrer S, Behr J, Stork V, Ramires M, Medard G, Frank O, [et al.]. Xanthohumol C. A minor bioactive hop compound: Production, purification strategies and antimicrobial test. Journal of Chromatography B. 2018;1095:39-49. https://doi.org/10.1016/j.jchromb.2018.07.018.
12. Salana L, Tofana M, Socaci S, Pop C, Michiu D, Farcas A. Determination of the volatile compounds from hop and hop products using ITEX/GC-MS Technique. Journal of Agroalimentary Processes and Technologies. 2012;18(2):110-115.
13. Fella P, Kaikiti K, Stylianou M, Agapiou A. HS-SPME-GC/MS analysis for revealing carob's ripening. Metabolites. 2022;12(7):656. https://doi.org/10.3390/metabo12070656.
14. Smallegange RC, Qiu YT, Bukovinszkine-Kiss G. The effect of aliphatic carboxylic acids on olfaction-based host-seeking of the malaria mosquito anopheles gambiae sensu stricto. Journal of Chemical Ecology. 2009;35:933-943. https://doi.org/10.1007/s10886-009-9668-7.
15. Podebrad F, Heil M, Reichert S, Mosandl A, Sewell A C, Bohles H. 4,5-Dimethyl-3-hydroxy-2[5H]-furanone (sotolone) - The odour of maple syrup urine disease. Journal of inherited metabolic disease. 1999;22(2):107-114.
16. Ashmawy NA, Salem MZM, EL-Hefny M, Abd El-Kareem MSM, El-Shanhorey NA, Mohamed AA, Salem ÀZM. Antibacterial activity of the bioactive compounds identified in three woody plants against some pathogenic bacteria. Microbial Pathogenesis. 2018;121:331-340. https://doi.org/10.1016/j.micpath.2018.05.032.
17. Imai H, Hotta O, Yoshimura M, Konta T. Deoxyspergualin, an immunosuppressant, in patients suffering from nephropathies with crescent formation: an open-label trial to evaluate safety and efficacy. Clinical and Experimental Nephrology. 2006;10(1):40-54. https://doi.org/10.1007/s10157-005-0396-1.
18. Caldas LA, Horvath RO, Ferreira-Silva GA, Ferreira MJP, Ionta M, Sartorelli P. Calein C, a sesquiterpene lactone isolated from Calea Pinnatifida (Asteraceae), inhibits mitotic progression and induces apoptosis in MCF-7 Cells. Frontiers Pharmacology. 2018;9:1191. https://doi.org/10.3389/fphar.2018.01191.
19. Asif M, Shafaei À, Fatemeh Jafari S, Kithur Mohamed S, Ezzat MO, Shah A, [et al.]. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT 116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicology Letters. 2016;257:84-96. https://doi.org/10.1016/j.toxlet.2016.05.027.
20. Goore SG, Ouattara ZA, Yapi AT. Chemical composition of the leaf oil of Artabotrys jollyanus from C?te d'Ivoire. Revista Brasileira de Farmacognosia. 2017;27(4):414-418. https://doi.org/10.1016/j.bjp.2017.04.001.
21. Nakamura S, Nakashima S, Tanabe G, Oda Y, Yokota N, Fujimoto K, [et al.]. Alkaloid constituents from flower buds and leaves of sacred lotus (Nelumbo nucifera, Nymphaeaceae) with melanogenesis inhibitory activity in B16 me­lanoma cells. Bioorganic & Medicinal Chemistry. 2012;21(3):779-787. https://doi.org/10.1016/j.bmc.2012.11.038.
22. Wilsdorf M, Reissig H-U. A Convergent Total Synthesis of the Telomerase Inhibitor (±)-?-Rubromycin. Angewandte Chemie. 2014;53(17):4332-4336. https://doi.org/10.1002/anie.201400315.
23. Tanabe M, Urano S. Biosynthetic studies with 13C: The antifungal antibiotic ilicicolin H. Tetrahedron. 1983;39(21):3569-3574. https://doi.org/10.1016/S0040-4020(01)88667-1.
24. Sunita A, Ganesh K. Gas Chromatography-Mass Spectrometry (GC-MS) determination of bioactive constituents from the methanolic and ethyl acetate extract of Cenchrus setigerus Vahl (Poaceae). The Pharma Innovation Journal. 2017;6(11):635-640.
25. Ekstrand E, Esposito D, Ragnarsson O, Isgaard J, Johannsson G. Metabolic effects of cortisone acetate vs hydrocortisone in patients with secondary adrenal insufficiency. Journal of the Endocrine Society. 2020;4(12):160. https://doi.org/10.1210/jendso/bvaa160.
26. Passmore AP, Whitehead EM, Crawford V, McVeigh GE, Johnston GD. The antihypertensive and metabolic effects of low and conventional dose cyclopenthiazide in type ii diabetics with hypertension. QJM: An International Journal of Medicine. 1991;81(2):919-928. https://doi.org/10.1093/oxfordjournals.qjmed.a068640.
27. Lopez-Munoz F, Alamo C, Garcia-Garcia P. The discovery of chlordiazepoxide and the clinical introduction of benzodiazepines: Half a century of anxiolytic drugs. Journal of Anxiety Disorders. 2011;25(4):554-562. https://doi.org/10.1016/j.janxdis.2011.01.002.
28. Masters PA, O'Bryan TA, Zurlo J, Miller DQ, Joshi N. Trimethoprim-Sulfamethoxazole Revisited. Archives of Internal Medicine. 2003;163(4):402-410. https://doi.org/10.1001/archinte.163.4.402.
29. Idan SA, Al-Marzoqi AH, Hameed IH. Spectral analysis and anti-bacterial activity of methanolic fruit extract of Citrullus colocynthis using gas chromatography-mass spectrometry. African Journal of Biotechnology. 2015;14(46):3131-3158. https://doi.org/10.5897/AJB2015.14957.
30. Rettberg N, Thoerner S, Labus AB, Garbe L-A. Aroma active monocarboxylic acids - origin and analytical characterization in fresh and aged hops. BrewingScience. 2014;67:33-47.
31. Takoi K, Itoga Y, Koie K, Takayanagi J, Kaneko T, Watanabe T, Nomura M. Behaviour of hop-derived branched-chain esters during fermentation and unique characteristics of Huell Melon and Ekuanot (HBC366) hops. BrewingScience. 2018;71:100-109.
32. Brendel S, Hofmann T, Granvogl M. Dry-hopping to modify the aroma of alcohol-free beer on a molecular level. Journal of Agricultural and Food Chemistry. 2020;68(32):8602-8612. https://doi.org/10.1021/acs.jafc.0c01907.
33. Brendel S, Hofmann T, Granvogl M. Hop-induced formation of ethyl esters in dry-hopped beer. Food Production, Processing and Nutrition. 2020;2:18. https://doi.org/10.1186/s43014-020-00030-0.
34. Piornos JA, Balagiannis DP, Methven L, Koussissi E, Brouwer E, Parker JK. Elucidating the odoractive aroma compounds in alcohol-free beer and their contribution to the worty flavor. Journal of Agricultural and Food Chemistry. 2020;68(37):10088-10096. https://doi.org/10.1021/acs.jafc.0c03902.
35. Vitalini S, Di Martile M, Cicaloni V, Iannone M, Salvini L, Del Bufalo D, [et al.]. Volatile and non-volatile content determination and biological activity evaluation of fresh Humulus lupulus L. (cv. Chinook) leaves and inflorescences. Separations. 2023;10:91. https://doi.org/10.3390/separations10020091.
36. Knudsen JT, Eriksson R, Gershenzon J, St?hl B. Diversity and distribution of floral scent. Botanical Review. 2006;72(1):1-120. http://www.jstor.org/stable/4354511.
37. Paguet AS, Siah A, Lefevre G, Moureu S, Cadalen T, Samaillie J, [et al.]. Multivariate analysis of chemical and genetic diversity of wild Humulus lupulus L. (hop) collected in situ in northern France. Phytochemistry. 2023;205:113508. https://doi.org/10.1016/j.phytochem.2022.113508.
38. Amer DA, Albadri AAM, El-Hamshary HA, Nehela Y, El-Hawary MY, Makhlouf AH, [et al.]. Impact of salting techniques on the physio-chemical characteristics, sensory properties, and volatile organic compounds of ras cheese. Foods. 2023;12:1855. https://doi.org/10.3390/foods12091855.
Zakharov Maxim A., Candidate of Technical Science,
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Zakharova Varvara A.,
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Gribkova Irina N., Candidate of Technical Science,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow,119021, Russia

Mikhailova I.Y.Honey Drinks and Prospects for Their Quality Control

P. 10-13 DOI: 10.52653/PIN.2023.04.004

Key words
honey, plant raw materials, honey drinks, mead, sbiten, quality control

The changing climate and the rapid deterioration of the environment in the 21st century pose a difficult task for people - through the possibilities of functional nutrition, to create conditions for providing the population with products with a high content of natural biologically active compounds that can support and preserve health. Drinks based on honey from numerous cultivated and wild plants from various regions, spicy and aromatic raw materials, produced on the basis of technological developments and scientific research in the food industry, preserving valuable components of raw materials - a promising direction of our time. Traditional Russian drinks, known back in ancient Rus': mead, a fermented drink with an ethanol content of no more than 6%, and sbiten, a non-alcoholic version of a honey drink, are at a new stage of popularity as sources of a wide range of substances beneficial to the body. The reduced ethanol content in mead and its absence in sbiten make them, in addition, a good alternative for the future transition to the population consuming predominantly low-alcohol drinks, preventing the development of alcohol dependence, which creates the basis for the further establishment of this direction as socially significant. An important condition for achieving the potential inherent in the synergistic effect of the components of honey and plant raw materials is both confirmation of the composition of the initial components in the product, as stated, and the preservation of their presence after completion of the production process - primarily heat treatment, which can reduce the enzymatic activity of honey in the product. A wide list of methods for monitoring the quality of honey, establishing the authenticity of its botanical composition, macro- and microelements, the composition of the vitamin complex and its compliance with safety indicators have been developed and applied. An important indicator of the value of a honey drink, combining the biological activity of honey and plants in the composition, is the indicator of its antioxidant activity. And confirmation of the botanical origin of honey, i. e. the presence in the composition of products of specific types of honey that differ in mineral, vitamin and organoleptic composition, can be performed by the DNA identification method, which examines the origin of pollen of honey plants.

1. Lesovskaya MI, Igoshin AS. National traditions and technological innovations in the production of honey drink. In book: Current issues of science and modern society. Penza: Nauka i Prosveshchenie, 2022. P. 207-251. (In Russ.).
2. Efanov MV. Innovative cavitation technology for producing functional drinks from wild plants. Mezhdunarodnyi zhurnal prikladnykh i fundamental"nykh issledovanii = International Journal of Applied and Fundamental Research. 2022; (2):72-75. https://doi.org/10.17513/mjpfi.13182. (In Russ.).
3. Korkacheva OV. Systematization of factors that form the consumer value of drinks developed on the basis of the concept of their socially significant properties. Dissertation abstract of candidate of technical sciences. Kemerovo, 2009. 20 p. (In Russ.)
4. Chekryga GP, Nitsievskaya KN., Golub OV., Motovilov OK. Sposob proizvodstva siropov na osnove meda [Method for the production of honey-based syrups]. Russia patent RU 2778844 C1. 2022.
5. Harrasov AA. Creation of an electronic database of honey-based products. Vestnik Bashkirskogo gosudarstvennogo pedagogicheskogo universiteta im. M. Akmully = Bulletin of the Bashkir State Pedagogical University named after. M. Akmulla. 2019; (1(49)):53-56. (In Russ.).
6. Eliseev MN, Emelyanova LK, Kosareva OA. Return of forgotten traditions. Vestnik akademii = Bulletin of the Academy. 2016;(4):70-76. (In Russ.).
7. Kiseleva TF. Conceptual approach to the development of functional fermented drinks. Pivo i napitki = Beer and beverages. 2006;(3):4-5. (In Russ.).
8. Petrova AS, Glushchenko LF, Lapteva NG, Laricheva KN. Formation of the quality of mead when replacing part of the honey in the recipe with sugar. Nauchnyi vestnik = Scientific Bulletin. 2016;(4(10)):97-104. https://doi.org/10.17117/nv.2016.04.097.
9. Ivanchenko OB, Borisova EV, Yakovleva IN. Alco- and genoprotective properties of a naturally fermented drink based on honey. Pivo i napitki = Beer and beverages. 2009;(2):38-41. (In Russ.).
10. Eliseev MN, Emelyanova LK. Development of a recipe for a concentrated base of a non-alcoholic drink of increased biological value. Ekonomika i upravlenie: problemy, resheniya = Economics and management: problems, solutions. 2018;3(12):194-200. (In Russ.).
11. Zavorokhina NV, Solovyova MP. Modern approaches to modeling recipes and technologies of national Russian drinks. Industriya pitaniya = Food industry. 2017;(2(3)):25-37. (In Russ.).
12. Panasyuk AL, Kuzmina EI, Sviridov DA, Ganin MYu. Individual integrated approach to honey identification using instrumental methods of analysis and statistical processing of results. Pishchevye sistemy = Food systems. 2023;6(2):211-223. https://doi.org/10.21323/2618-9771-2023-6-2-211-223. (In Russ.).
13. Gruznova OA, Lobanov AV, Sokhlikov AB, Gruznov DV. Vliyanie vysokikh i nizkikh temperatur na soderzhanie 5-gidroksimetilfurfurola v mede [The influence of high and low temperatures on the content of 5-hydroxymethylfurfural in honey]. Sbornik nauchno-prakticheskikh materialov Mezhdunarodnoi nauchno-prakticheskoi konferentsii: Fundamental'nye i prikladnye resheniya prioritetnykh zadach pchelovodstva. 2023, p. 82-87. (In Russ.).
14. Yesenkina SN. Features of the mine­ral composition of honeys of different botanical origin. Vestnik Ryazanskogo gosudarstvennogo agrotekhnologicheskogo universiteta im. P.A. Kostycheva = Bulletin of the Ryazan State Agrotechnological University named after P.A. Kostycheva. 2022;14(2):42-49. https://doi.org/10.36508/RSATU.2022.54.2.005. (In Russ.).
15. Popkova MA. The influence of the botanical origin of honey on the content of water-soluble vitamins in it. Sbornik nauchnykh trudov Krasnodarskogo nauchnogo tsentra po zootekhnii i veterinarii = Collection of scientific papers of the Krasnodar Scientific Center for Animal Science and Veterinary Medicine. 2020;9(1):299-302. https://doi.org/10.34617/121x-gb63. (In Russ.).
16. Makarova NV, Limanova VS, Bordinova VP. Antioxidant substances of different varieties of honey. Izvestiya vysshikh uchebnykh zavedenii. Pishchevaya tekhnologiya = News of higher educational institutions. Food technology. 2011;(1(319)): 18-20. (In Russ.).
17. Chugunova OV, Grashchenkov DV., Vyat­kin AV. Application of fruit and berry raw materials in the formulations of hot drinks. Nauchnyi zhurnal NIU ITMO. Seriya: Protsessy i apparaty pishchevykh proizvodstv = Scientific journal of NRU ITMO. Series: Processes and apparatus for food production. 2020;(4(46)):39-52. https://doi.org/10.17586/2310-1164-2020-10-4-39-52. (In Russ.).
18. Bodorev MM. Improving the assessment of consumer properties of alcoholic and non-alcoholic drinks based on the determination of antioxidant activity. Dissertation abstract of candidate of technical sciences. Moscow, 2009. 24 p. (In Russ.).
19. Maslova IN, Nesterov ED, Moiseyak MB. Study of the influence of spicy-aromatic raw materials on the quality of honey drinks. Stolypinskii vestnik = Stolypin Bulletin. 2022;4(3):46. (In Russ.).
20. Vafin RR, Mikhailova IYu, Ageikina II, Sviridov DA, Ganin MYu. Modeling DNA technology for determining the botanical origin of honey. Pishchevaya promyshlennost' = Food processing industry. 2023;(11):72-75. https://doi.org/10.52653/PPI.2023.11.11.015. (In Russ.).
Mikhailova Irina Y.,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow, 119021, Russia

Ermolaev S.V.Efficient Pressing of Grapes on a Closed Pneumatic Press

P. 14-19 DOI: 10.52653/PIN.2023.04.005

Key words
press, membrane, grapes, wort, pulp, inert gas

The article discusses modifications of pneumatic presses with an elastic membrane for pressing grapes, pulp, fruits and berries. The principle of operation of the presses is described; the configuration of the internal elastic pipe. The parameters of the drum operation (operating pressure, the number of tension-drop cycles of the membrane, the number of revolutions of the drum, etc.) are set from the control panel to a logic controller that interacts with a pressure switch, an electric motor for rotation and a compressor. The limit pressure values, the number of pressing cycles, the increase in pressure from cycle to cycle, the total spin time and additional cycles to enhance the spin can also be entered into the logic controller. Using the example of Puleo presses, compression in an atmosphere of an inert gas, nitrogen, is described, which reduces the oxidation of the resulting wort. The technical characteristics of the presses of the SC and SF series are given - presses with a closed tank and with a perforated one. The order of operation of the press is described. The characteristics of the wort obtained from white grapes with the application of different pressures are given. It was found that when applying higher pressure, the juice yield is higher, the pressing time is shorter, the acidity is lower, and the content of polyphenols and suspended particles is higher. Data on the quality of white wine from wort obtained on such a press, but in an inert gas atmosphere, are presented. There is a high intensity of some flavors. The use of an elastic membrane in the press ensures an even distribution of the product in the drum and a large area of the juice transfer surface. After pneumatic pressing, the high quality of the juice is high, since the raw material is not subjected to abrasion by working bodies during pressing, and the radial direction of pressure from the center does not compact the material, but on the contrary, "stretches" it as it moves away from the center. Pneumatic presses are used in wineries to produce high-quality wort for fine wines.

1. Ermolaev SV, Krivovoz AG, Talalai OA. New pneumatic press with elastic central membrane. Vinodelie i vinogradarstvo = Winemaking and viticulture. 2008;(1):28-29. (In Russ.).
2. Prexa N - INFINITY. Close Tank Pneumatic Press - Infinity [Internet]. [cited 2023 October 5]. URL: https://www.puleoitalia.com/en/close-tank-pneumatic-press-infinity/prexa-n-infinity.
3. Ermolaeva GA. Spravochnik rabotnika laboratorii pivovarennogo predprijatija [Brewery Lab Employee Handbook]. Saint-Petersburg: Professija, 2004. 536 p. (In Russ.).
4. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. American Journal of Enology and Viticulture. 1965;16(3):144-158. https://doi.org/10.5344/ajev.1965.16.3.144.
5. Catania P, Comparetti A, Morello G, Orlando S, Vallone M. Pneumatic press equipped with the vortex system for white grapes processing: first results. Chemical Engineering Transactions. 2019;75;73-78. https://doi.org/10.3303/CET1975013.
Ermolaev Sergey V., Candidate of Technical Science,
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4, 4/22, Kosmodamianskaya embankment, Moscow, 115035, Russia


Shanenko E.F., Skorodumov A.S., Mukhamedzhanova T.G., Nesterov E.D.Preparation of Fructooligosaccharide Concentrate from Jerusalem Artichoke Tubers for Functional Products

P. 20-25 DOI: 10.52653/PIN.2023.04.007

Key words
inulin, fructooligosaccharides, Jerusalem artichoke, prebiotics, functional drinks, concentrates, enzymatic hydrolysis

Jerusalem artichoke is a natural source of inulin and fructooligosaccharides, which have a wide range of beneficial properties for the human body. Fructooligosaccharides and inulin are prebiotic substances that promote the growth of beneficial microflora in the intestine, as well as increase the nutritional value of foods and help improve digestion. The purpose of this work was to study methods for obtaining Jerusalem artichoke hydrolysates with a given degree of polymerization of fructooligosaccharides. The objects of the study were Jerusalem artichoke of the "Interest" variety of the autumn 2023 harvest, grown in the Astrakhan region, and the enzyme preparation "Invertase" produced by Biopreparat LLC. The results showed that the use of an enzyme preparation affects the average degree of polymerization of Jerusalem artichoke fructooligosaccharides and the selection of parameters of enzymatic hydrolysis allows to obtain hydrolysates with a given degree of polymerization.

1. Afoakwah N. A., Mahunu G.K. Utilization of Jerusalem artichoke (Helianthus tuberosus L.) tuber as a prebiotic and a symbiotic. In book: African Fermented Food Products - New Trends. New York: Springer Cham, 2022. P. 525-536. https://doi.org/10.1007/978-3-030-82902-5_35.
2. Redondo-Cuenca A, Herrera-V?zquez SE, Condezo-Hoyos L, G?mez-Ord??ez E, Rup?rez P. Inulin extraction from common inulin-containing plant sources. Industrial crops and products. 2021;170:113726. https://doi.org/10.1016/j.indcrop.2021.113726.
3. Serbaeva ER, Yakupova AB, Magasumova YuR, Farkhutdinova KA, Akhmetova GR, Kuluev BR. Inulin: natural sources, features of metabolism in plants and practical application Biomics. 2020;12(1):57-79. https://doi.org/10.31301/2221-6197. bmcs.2020-5. (In Russ.).
4. Kherade M, Solanke S, Tawar M, Wankhede S. Fructooligosaccharides: A comprehensive review. Journal of ayurvedic and herbal medicine. 2021;7(3):193-200. https://doi.org/10.31254/jahm.2021.7305.
5. Bedzo OKK., van Rensburg E, Gorgens JF. Investigating the effect of different inulin-rich substrate preparations from Jerusalem artichoke (Helianthus tuberosus L.) tubers on efficient inulooligosaccharides production. Preparative Biochemistry & Biotechnology. 2021;51(5):440-449. https://doi.org/10.1080/10826068.2020.1827429.
6. Nyarko G, Afoakwah NA, Dong Y. Non-thermal inactivation of polyphenoloxidase from jerusalem artichoke (Helianthus tuberusus l.). UDS international journal of development. 2019;6(1):62-71. https://doi.org/10.47740/330.UDSIJD6i.
7. GOST 28561-90. Fruit and vegetable processing products. Methods for the determination of dry substances or moisture. Moscow: Standartinform, 2011. 75 p. (In Russ.).
8. Krivorotova T, Sereikaite J. Seasonal changes of carbohydrates composition in the tubers of Jerusalem artichoke. Acta physiologiae plantarum. 2014;36:79-83. https://doi.org/10.1007/s11738-013-1388-5.
9. Rubel IA, Iraporda C, Manrique GD, Genovese DB, Abraham AG. Inulin from Jerusalem artichoke (Helianthus tuberosus L.): From its biosynthesis to its application as bioactive ingredient. Bioactive carbohydrates and dietary fibre. 2021;26:100281. https://doi.org/10.1016/j.bcdf. 2021.100281.
10. Kozhukhova M, Nazarenko M, Barkhatova T, Khripko I. Obtaining and identification of inulin from Jerusalem artichoke (Helianthus tuberosus) tubers. Foods and Raw materials. 2015. Ò. 3, Iss. 2. P. 13-22. https://doi.org/10.12737/13115.
11. Bilenka I, Lazarenko N, Zolovska O, Golinskaya Ya. Features of the enzyme composition of Jerusalem artichoke tubers. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. 2019;21(91):14-19. https://doi.org/10.32718/nvlvet-f9103.
12. Zhang L, Liu W, Ji J, Deng L, Feng Q, Shi W, Gao J. Inactivation of inulinase and marination of High-Quality Jerusalem Artichoke (Helianthus tuberosus L.) pickles with screened dominant strains. Frontiers in bioengineering and biotechnology. 2021;8:626861. https://doi.org/10.3389/fbioe.2020.626861.
Shanenko Elena F., Candidate of Biological Science, Àssociate Professor,
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Muhamedzhanova Tat'yana G., Candidate of Technical Science,
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Russian Biotechnological University (ROSBIOTECH),
11, Volokolamskoe highway, Moscow, 125080, Russia
Skorodumov Aleksandr S.,
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Nesterov Egor D.,
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LLC "Bavar+",
2, 2nd Mytishchinskaya Str., Moscow, 129626, Russia

Skorodumov A.S., Shanenko E.F., Moiseyak M.B., Grushnikova V.I., Manin E.S.The Effect of Extraction Parameters on the Yield of Phenolic Compounds from Hops

P. 26-30 DOI: 10.52653/PIN.2023.04.006

Key words
Humulus lupulus, common hops, extraction, phenols, antioxidants

Hops (Humulus lupulus), used mainly in brewing, contains a large amount of biologically active substances of a phenolic nature. During various technological processes, these substances may lose their properties, therefore, the development of methods for obtaining extracts with a high content of non-oxidized phenolic compounds is relevant. In this work, the influence of the main extraction parameters, such as the concentration of the extractant, the ratio of hop mass to extractant volume, temperature and duration of extraction on the yield of antioxidants was studied.

1. The State Pharmacopoeia of the Russian Federation XIV edition [Internet]. [cited 2023 October 5]. URL: https://pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-14/?ysclid=lq2lvxoumn930543865.
2. European Pharmacopoeia Tenth Edition. Volume I [Internet]. [cited 2023 October 5]. URL: https://www.nihs.go.jp/dnfi/pdf/RI_PDF/EP1-1.pdf.
3. Bolton JL, Dunlap TL, Hajirahimkhan A, Mbachu O, Chen SN, Chadwick L, [et al.]. The multiple biological targets of hops and bioactive compounds. Chemical Research in Toxicology. 2019;32(2):222-233. https://doi.org/10.1021/acs.chemrestox.8b00345.
4. Karabin M, Hudcova T, Jelinek L, Dostalek P. Biologically active compounds from hops and prospects for their use. Comprehensive reviews in food science and food safety. 2016;15(3):542-567. https://doi.org/10.1111/1541-4337.12201.
5. Kashyap D, Sharma A, Tuli HS, Sak K, Punia S, Mukherjee TK. Kaempferol - A dietary anticancer molecule with multiple mechanisms of action: Recent trends and advancements. Journal of Functional Foods. 2017;30:203-219. https://doi.org/10.1016/j.jff.2017.01.022.
6. Semwal DK, Semwal RB, Combrinck S, Viljoen A. Myricetin: a dietary molecule with diverse biological activities. Nutrients. 2016;8(2):90. https://doi.org/10.3390/nu8020090.
7. Anand David AV, Arulmoli R, Parasuraman S. Overviews of Biological Importance of Quercetin: A Bioactive Flavonoid. Pharmacognosy Reviews. 2016;10(20):84-89. https://doi.org/10.4103/0973-7847.194044.
8. Chesnokova AN, Lutsky VI. Prenylchalcones of hop (humulus lupulus l.): isolation, structure, aspects of application. Izvestiya Irkutskogo gosudarstvennogo universiteta. Seriya: Biologiya. Ekologiya. 2008;1(2):94-96. (In Russ.).
9. Zanoli P, Zavatti M. Pharmacognostic and pharmacological profile of Humulus lupulus L. Journal of Ethnopharmaco­logy. 2008;116(3):383-396. https://doi.org/10.1016/j.jep.2008.01.011.
10. Paszkot J., Kawa-Rygielska J., Aniol M. Properties of dry hopped dark beers with high xanthohumol content. Antioxidants. 2021;10(5):763. https://doi.org/10.3390/antiox10050763.
11. Elrod SM. Xanthohumol and the medicinal benefits of beer. In book: Polyphenols: mechanisms of action in human health and di­sease. Academic Press, 2018, pp. 19-32. https://doi.org/10.1016/B978-0-12-813006-3.00003-9.
12. Drenzek JG, Seiler NL, Jaskula-Sztul R, Rausch MM, Rose SL. Xanthohumol decreases Notch1 expression and cell growth by cell cycle arrest and induction of apoptosis in epithelial ovarian cancer cell lines. Gynecologic Oncology. 2011;122:396-401. https://doi.org/10.1016/j.ygyno.2011.04.027.
13. Ven? R, Benelli R, Minghelli S, Astigiano S, Tosetti F, Ferrari N. Xanthohumol impairs human prostate cancer cell growth and invasion and diminishes the incidence and progression of advanced tumors in TRAMP Mice. Molecular Medicine. 2012;18:1292-1302. https://doi.org/10.2119/molmed.2012.00174.
14. Arnaiz-Cot JJ, Cleemann L, Morad M. Xanthohumol modulates calcium signaling in rat ventricular myocytes: possible antiarrhythmic properties. Journal of Pharmacology and Experimental Therapeutics. 2017;360(1):239-248. https://doi.org/10.1124/jpet.116.236588.
15. Bolca S, Li J, Nikolic D, Roche N, Blon­deel Ph, Possemiers S, [et al.]. Disposition of hop prenylflavonoids in human breast tissue. Molecular Nutrition & Food Research. 2010;54(S2):S284-S294. https://doi.org/10.1002/mnfr.200900519.
16. Calvo-Castro LA, Burkard M, Sus N, Scheubeck G, Leischner Ch, Lauer UM, [et al.]. The oral bioavailability of 8-prenylnaringenin from hops (Humulus Lupulus L.) in healthy women and men is significantly higher than that of its positional isomer 6-prenylnaringenin in a randomized crossover trial. Molecular Nutrition Food Research. 2018;62(7):1700838. https://doi.org/10.1002/mnfr.201700838.
17. Pohjanvirta R, Nasri A. The potent phytoestrogen 8-prenylnaringenin: a friend or a foe? International Journal of Molecular Sciences. 2022;23(6):3168. https://doi.org/10.3390/ijms23063168.
18. Ono M, Yamaguchi N, Yamaguchi K. Xanthohumol-enriched hop extract US8142821B2. Date of patent: Mar. 27, 2012.
Shanenko Elena F., Candidate of Biological Science, Àssociate Professor,
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Moiseyak Marina B., Candidate of Technical Science, Professor,
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Russian Biotechnological University (ROSBIOTECH),
11, Volokolamskoe highway, Moscow, 125080, Russia
Grushnikova Varvara I.;
Manin Egor S.,
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Russian Biotechnological University (ROSBIOTECH),
11, Volokolamskoe highway, Moscow, 125080, Russia;
LLC "Bavar+",
2, 2nd Mytishchinskaya Str., Moscow, 129626, Russia
Skorodumov Aleksandr S.,
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LLC "Bavar+",
2, 2nd Mytishchinskaya Str., Moscow, 129626, Russia


Eliseev M.N., Kosareva O.A.The Sencha Tea Quality Sold on the Russian Market

P. 31-35 DOI: 10.52653/PIN.2023.04.002

Key words
tea, varieties, regions of growth, phenolic compounds, caffeine, nitrogen compounds, sugars

The article is devoted to the sencha variety green tea quality. The composition of tea organic compounds is given, indicating the regions of harvest. We studied samples of sencha green tea, packaged and sold in Russia with the collection location of sample 1 in China, and 2 and 3 in Japan. To achieve the studies goal - to investigate the composition of sencha tea organic compounds that affect organoleptic characteristics, depending on the region of growth - determination methods accepted in the industry, including HPLC, were used. It was shown that in terms of the content of water-soluble extractive substances, tea samples 1 and 2 met the requirements of GOST 32574-2013, in contrast to the level of water-soluble extractive substances in sample 3, which correlated with the data of organoleptic analysis. The authors established the protein content in green tea in the range of 2507.9-2577.4 mg/100 g of tea or 2.5-2.6%, which distinguishes it from other harvesting regions. The amine nitrogen content in samples 1-3 ranged from 5.3-6.8 g/100g or 5.3-6.8%, which is close to the level of values for Chinese tea varieties. The level of total phenolic compounds in the samples was comparable to those previously stated and amounted to 4.9-5.3%. The authors noted that the content of catechins in the studied samples was in the range of 20.5-23.5%, which is higher than the declared quantities, and the content of tannins in the studied samples was in the range of 0.54-0.78%, which is lower than the declared levels. Of the monomeric phenolic compounds, gallic acid was predominantly identified. A correlation was noted between the ratio of phenolic and nitrogen compounds with organoleptic indicators. The caffeine levels in the samples were 2.3-2.4%, which is comparable to other researchers and consistent with the Chinese harvest region. Thus, it was concluded that the criteria for identifying the harvest region may be the content of caffeine and nitrogen compounds.

1. Sajilata MG, Bajaj PR, Singhal RS. Tea polyphenols as nutraceuticals. Comprehensive reviews in food science and food safety. 2008;7(3):229-254.
2. Hasegawa T, Shimada Y, Saito H, Fujihara T, Haraguchi K, [et al.]. Characteristic aroma features of Tencha and Sencha green tea leaves manufactured by different processes. Natural Product Communications. 2016;11:1171-1173. https://doi.org/10.1177/1934578X1601100835.
3. Yashin A, Nemzer B, Combet EY, Yashin Y. Determination of the chemical composition of tea by chromatographic methods: a review. Journal of Food Research. 2015;4(3):56-88. https://doi.org/10.5539/jfr.v4n3p56.
4. Masek A, Chrzescijanska E, Kosmalska A, Zaborski M. Antioxidant activity determination in Sencha and Gun Powder green tea extracts with the application of voltammetry and UV-VIS spectrophotometry. Comptes Rendus Chimie. 2012;15(5):424-427. https://doi.org/10.1016/j.crci.2012.01.005.
5. GOST 32574-2013. The green tea. Technical specifications. Ìoscow: Standartinform, 2012. 14 p. (In Russ.).
6. GOST R ISO 9768-2011. Tea. The watersoluble extractive substances determination methods. Ìoscow: Standartinform, 2010. 8 p. (In Russ.).
7. GOST R ISO 14502-1-2010. Tea. Method for determining total polyphenol content. Ìoscow: Standartinform, 2019. 12 p. (In Russ.).
8. Salawu SO, Olukemi BE, Asikhia IC, Akindahunsi AA. Mineral elements bio-accessibility and antioxidant indices of blanched basella rubra at different phases of in vitro gastrointestinal digestion. Preventive Nutrition and Food Science. 2018;23(1):22-29. https://doi.org/10.3746/pnf.2018.23.1.22.
9. GOST 19885-74. Tea. Methods for determining tannin and caffeine content. Ìoscow: Standartinform, 2009. 5 p. (In Russ.).
10. Nelum P. Piyasena KG, Hettiarachchi LSK, Jayawardhane SADPS, Edirisinghe ENU, Jayasinghe WS. Evaluation of inherent fructose, glucose and sucrose concentrations in tea leaves (Camellia sinensis L.) and in black tea. Applied Food Research 2022;2(1):100100. https://doi.org/10.1016/j.afres.2022.100100.
11. Maltsev PM, Velikaya EI, Kolotusha PV. Chemical-technological control of malt and beer production [Khimiko-tekhnologicheskiy kontrol' proizvodstva soloda i piva]. Moscow: Food industry. 1976. 448 p. (In Russ.).
12. GOST 34789-2021. Brewing products. Identification. Determination of the mass concentration of total nitrogen by the Kjeldahl method. Moscow: Standartinform, 2021. 14 p. (In Russ.).
13. Graham HG. Green tea composition, consumption, and polyphenol chemistry. Preventive Medicine. 1992;21(3):334-350. https://doi.org/10.1016/0091-7435(92)90041-F.
14. Guo Z, Barimah AO, Yin L, Chen Q, Shi J, El-Seedi HR. Intelligent evaluation of taste constituents and polyphenols-to-amino acids ratio in matcha tea powder using near infrared spectroscopy. Food Chemistry. 2021;353:129372. https://doi.org/10.1016/j.foodchem.2021.129372.
15. Sakasegawa M, Yatagai M. Composition of pyrolyzate from Japanese green tea. Journal of Wood Science. 2005;51:73-76. https://doi.org/10.1007/s10086-003-0613-z.
16. Chun JU, Choi J, Lim KC, Kim YJ. Classification of Korean green tea products based on chemical components. Korean Journal of Crop Science. 2004;49(4):295-299.
17. Wei K, Ruan L, Li H, Wu L, Wang L, Cheng H. Estimation of the effects of major chemical components on the taste quality of green tea. International Food Research Journal. 2019;26(3):869-876.
18. Yanagimoto K, Ochi H, Lee K-G, Shibamoto T. Antioxidative Activities of Volatile Extracts from Green Tea, Oolong Tea, and Black Tea. Journal of Agricultural and Food Chemistry. 2003;51(25):7396-7401. https://doi.org/10.1021/jf030127i.
19. Khasnabis J, Rai Ch, Roy A. Determination of tannin content by titrimetric method from different types of tea. Journal of Chemical and Pharmaceutical Research. 2015;7(6):238-241.
20. Liu Q, Zhang YJ, Yang CR, Xu M. Phenolic antioxidants from green tea produced from Camellia crassicolumna Var. multiplex. Journal of Agricultural and Food Chemistry. 2009;57(2):586-590.
21. Zhao CN, Tang GY, Cao SY, Xu XY, Gan RY, Liu Q, [et al.]. Phenolic profiles and antioxidant activities of 30 tea infusions from green, black, oolong, white, yellow and dark teas. Antioxidants (Basel). 2019;8(7):215. https://doi.org/10.3390/antiox8070215.
22. Ramdani D, Chaudhry AS, Seal SJ. Alkaloid and polyphenol analysis by HPLC in green and black tea powders and their potential use as additives in ruminant diets. The 1st International Conference and Exhibition on Powder Technology Indonesia (ICePTi). 2017;1927:030008. https://doi.org/10.1063/1.5021201.
23. Shevchuk A, Megias-Perez R, Zemedie Y, Kuhnert N. Evaluation of carbohydrates and quality parameters in six types of commercial teas by targeted statistical analysis. Food Research International. 2020;133:109122. https://doi.org/10.1016/j.foodres.2020.109122.
Eliseev Michail N., Doctor of Technical Science, Professor,
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Russian University of Economics named after G.V. Plekhanov,
36, Stremyanny lane, Moscow, 117997, Russia
Kosareva Olga A., Candidate of Technical Science, Associate Professor,
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Moscow Financial and Industrial University "Synergy",
9/14, bldg. 1, Meshchanskaya str., Moscow, 129090, Russia

Karpenko D.V., Matveev S.V., Morenkov N.V., Morozov D.A., Mamonova A.S. Enzyme Preparations in Malt Production and Brewing: a Range of Tasks to be Solved. Part I

P. 36-41 DOI: 10.52653/PIN.2023.04.001

Key words
malting production, enzyme preparations, intensification of malting, improvement of malt quality, acceleration of wort and beer filtration

The article systematizes and analyzes information about enzyme preparations of various origins and types of action, proposed for use to intensify technological processes at the stages of malting and brewing production. The advantages provided by the rational use of enzyme preparations are presented. The first part of the article discussed the use of enzyme preparations to intensify the technological stages of malting production. The stages at which the implementation of the discussed technique is recommended are indicated. A number of enzyme preparations recommended for use in the production of brewing malt are listed, and recommended dosages for their application are given. They provide a significant reduction in the duration of individual stages, primarily the malting stage, and make it possible to use grain with low characteristics as raw materials. It has been shown that the use of enzyme preparations, individually or in various combinations, makes it possible to increase the activity of a number of technologically important hydrolases of finished malt, its degree of dissolution, extractivity, and reduce glassiness. It has been noted that beer wort from malt produced using exogenous biocatalysts has a lower viscosity and a higher content of total extract and nutrients, including amine nitrogen. The possibility of intensification through the use of enzyme preparations for the production of dark barley malt, including those with increased amylase activity, has been demonstrated. Brief information is presented on the technology of fermented oat malt, the intensification of which is provided by an enzyme preparation of a complex type of action. The possibility of increasing the activity of hydrolases in the mass of germinating grains through the use of a starter yeast culture is mentioned. In conclusion, it is noted that most of the enzyme preparations discussed are produced by companies from unfriendly countries, which creates uncertainty with their availability in the Russian Federation in the current geopolitical situation.

1. Evans DE, Stewart S, Stewart D, Han Zh, Han Y, Able J.A. Profiling malt enzymes related to impact on malt fermentability, lautering and beer filtration performance of 94 commercially produced malt batches. Journal of the American Society of Brewing Chemists. 2022;80 (4):413-426. https://doi.org/10.1080/03610470.2021.1979891.
2. Svozil K. Experience with the application of Soviet enzymatic preparations in brewing. Kvasny prumysl. 1983;29 (2):26-28.
3. Abramovic B. Substitution of malt by maize and barley in beer production. Kvasny prumysl. 1983;29(9):200-205.
4. Cooper C, Evans D, Yousif A, Metz N, Koutoulis A. Comparison of the impact on the performance of small-scale mashing with different proportions of unmalted barley, Ondea Pro®, malt and rice. Journal of the Institute of Brewing. 2016;122 (2):218-227. https://doi.org/10.1002/jib.325.
5. Kato M, Kamada T, Mochizuki M, Sasaki T, Fukushima Y, Sugiyama T, [et al.]. Influence of high molecular weight polypeptides on the mouthfeel of commercial beer. Journal of the Institute of Brewing. 2021;127(1):27-40. https://doi.org/10.1002/jib.630.
6. Albini PA, Briggs DE, Wadeson A. Microbial enzymes and their effects on extract recoveries from unmalted adjuncts. Journal of the Institute of Bre­wing. 1987;93(2):97-104. https://doi.org/10.1002/j.2050-0416.1987.tb04483.x.
7. O'Connor B, Kerpes R, Titze J, Jacob F, Arendt E. Impact of various levels of unmalted oats (Avena sativa L.) on the quality and processability of mashes, worts, and beers. Journal of the American Society of Brewing Chemists. 2012;70(3):142-149.
8. Van Donkelaar LHG, Mostert J, Zisopoulos FK, Boom RM, van der Goot A-J. The use of enzymes for beer brewing: Thermodynamic comparison on resource use. Energy. 2016;115(1):519-527. https://doi.org/10.1016/j.energy.2016.09.011.
9. Bamforth C. Current perspectives on the role of enzymes in brewing. Journal of Cereal Science. 2009;50 (3):353-357. https://doi.org/10.1016/j.jcs.2009.03.001.
10. DuPont Brewing Solutions [Internet]. [cited 2023 August 27]. URL: https://xn-- 80adfdatufkwf1au8huc.xn--p1ai/files/misc/alphalase_advance_4000.pdf.
11. Gomaa AM. Application of enzymes in brewing. Journal of Nutrition and Food Science Forecast. 2018;1(1):1002. https://doi.org/10.5281/zenodo.3336203.
12. Tolkacheva AA, Cherenkov DA, Korneeva OS, Ponomarev PG. [Enzymes of industrial purpose - review of the market of enzyme preparations and prospects for its development]. Proceedings of the Voronezh State University of Engineering Technologies. 2017;79 (4):197-203. https://doi.org/10.20914/2310-1202-2017-4-197-203. (In Russ.).
13. Kotlar C, Ponce A, Roura S. Characterization of a novel protease from Bacillus cereus and evaluation of an eco-friendly hydrolysis of a brewery byproduct. Journal of the Institute of Brewing. 2015;121(4):558-565. https://doi.org/10.1002/jib.257.
14. Forssell P, Kontkanen H, Schols HA, Hinz S, Eijsink VGH, Treimo J, [et al]. Hydrolysis of brewers' spent grain by carbohydrate degrading enzymes. Journal of the Institute of Brewing. 2008;114(4):306-314.
15. Iliev I, Tchorbanov B, Todorova V. Enzymic protein hydrolysates from malt sprouts. Journal of the Institute of Brewing. 1992;98(2):139-142.
16. Walker SL, Fourgialakis M, Cerezo B, Livens S. Removal of microbial biofilms from dispense equipment: the effect of enzymatic pre-digestion and detergent treatment. Journal of the Institute of Brewing. 2007;113(1):61-66.
17. Todorova V. Intensification of malting using bioregulation. Kvasny prumysl. 1984;30(2):29-31.
18. Grujic O. Application of commercial enzyme preparation in the barley malting process. Journal of the Institute of Brewing. 1998;104 (5):249-253.
19. Ermolaev SV, Shishkina EI, Baholdina AD, Janenko DV, Leonova OP, Korablev RV. Prorashhivanie soloda s dobavleniem fermentnyh preparatov [Germination of malt with the addition of enzyme preparations]. Sbornik materialov nauchno-prakticheskoj konferencii molodyh uchenyh i specialistov: Aktual'nye voprosy povyshenija kachestva i bezopasnosti produktov pitanija. 2016, p. 75-77. (In Russ.).
20. Damdinsurjen A. Development of malt technology with the use of enzyme pre­parations for the production of beer with the addition of water extracts. Dissertation Abstract. Voronezh: Voronezh State Technological Academy, 2005. (In Russ.).
21. Damdinsurjen A, Faradzheva ED, Vostrikov SV. Enzyme preparations during ma­nufacture of light brewing malt. Pivo i napitki = Beer and beverages. 2003;(6):22-23. (In Russ.).
22. Kazakova EA, Ermolaeva GA. Intensification of malt growing during usage of apsoubtiline and of salts of magnesium and iron. Pivo i napitki = Beer and beverages. 2004;(4):32-33. (In Russ.).
23. Ermolaeva GA, Budakova JeD. Obtai­ning brewing malt using biocatalysts from barley of the Republic of Bashkortostan. Pivo i napitki = Beer and beverages. 2008; (5):34-35. (In Russ.).
24. Budakova JeD. Development of intensive technological methods for obtaining brewing malt from barley of the Republic of Bashkortostan using scarification and biocatalytic treatment. Dissertation Abstract. Moscow: Moscow State University of Food Production, 2008. (In Russ.).
25. Kazakova EA, Ermolaeva GA. Germination of barley with usage of chloride of calcium and enzyme preparation. Pivo i napitki = Beer and beverages. 2004;(2):30-31. (In Russ.).
26. Minhodzhov SN, Ermolaeva GA. The influence of biocatalysts on the quality of malt from barley of the Republic of Tajikistan. Pivo i napitki = Beer and beverages. 2009;(1):46-48. (In Russ.).
27. Minhodzhov SN, Ermolaeva GA. Improving the quality of malt from barley of the Republic of Tajikistan with the enzyme preparation Beerzym BG Super. Pivo i napitki = Beer and beverages. 2008;(4):64-65. (In Russ.).
28. Chanchikova AA, Kamenskaya EP. Research of influence of enzyme drugs on indicators of quality of light barley malten. Tekhnologiya i tovarovedenie innovatsionnykh pishchevykh produktov = Technology and merchandising of the innovative foodstuff. 2020;(5(64)):17-22. https://doi.org/10.33979/2219-8466-2020-64-5-17-22. (In Russ.).
29. Gribkova IN. Development of malt technology using biocatalysts. Dissertation Abstract. Moscow: Moscow State University of Food Production, 2006. (In Russ.).
30. Ermolaev SV. The use of Amyloprotoorisin for the production of dark diastatic malt. Pivo i napitki = Beer and beverages. 2008;(5):28-29. (In Russ.).
31. Agafonov GV, Chusova AE, Zelen'kova AV, Plotnikov VE. Effect of enzyme preparation ceramics 6xmg on indicators of oat malt quality. Vestnik Voronezhskogo gosudarstvennogo universiteta inzhenernykh tekhnologii = Proceedings of the Voronezh State University of Engineering Technologies. 2018;80(3):128-133. (In Russ.). https://doi.org/10.20914/2310-1202-2018-3-128-133.
32. Piegza M, Witkowska D, Stempniewicz R. Enzymatic and molecular characteristics of Geotrichum candidum strains as a starter culture for malting. Journal of the Institute of Brewing. 2014;120 (4):341-346. https://doi.org/10.1002/jib.167.
33. Dziuba E, Wojtatowicz M, Stempniewicz R, Foszczy?ska B. The use of Geotrichum candidum starter cultures in malting of brewery barley. Progress in Biotechnology. 2000;17:311-316. https://doi.org/10.1016/S0921-0423(00)80086-6.
34. Piegza M, Witkowska D, Robak M. Hydrolases of Geotrichum candidum yeasts in barley beta-glucan degradation. Electronic Journal of Polish Agricultural Universities. 2006;9(4):17.
Karpenko Dmitry V., Doctor of Technical Science, Professor,
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Matveev Sergej V.,
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Morenkov Nikolaj V.,
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Morozov Dmitry A.,
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Mamonova Anastasija S.,
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Moscow State University of Food Productions,
11, Volokolamskoye highway, Moscow, 125080, Russia


Results. "Glass Forum - Expert Club", Suzdal

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Student Competition "Clockwork Orange - 2023"