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Rambler's Top100

Beer and beverages №3/2023


Solov'eva S.Yu., Khlynovskii M.D.Applying of Malt-grain Concentrate Brand "Brownish" in Production of Beer and Beer Drinks

P. 4-7 DOI: 10.52653/PIN.2023.03.03.001

Key words
beer, malt-grain concentrate, colour, mouth feel, foam stability

Malt-grain concentrate is a multifunctional component in the production of beer and beer drinks, which allows you to adjust the color, taste and foam of beverages. The use of Brownish and Brownish Strong malt-grain concentrate in the recipes makes it possible to obtain new types of light and dark beers and drinks with a balanced taste and color. In terms of quality, such drinks meet the requirements of regulatory documentation for fermented drinks and have improved properties in terms of fullness of taste, foam stability and color intensity. The use of malt-grain concentrate provides an increase in the economic efficiency of enterprises - producers of beer and fermented beverages.

1. BJCP Special Ingredient Descriptions [Electronic resource]. URL: https://legacy.bjcp.org/docs/Ingredients.pdf (Accessed 03.02.2023).
2. Brewing handbook. Novozymes. Version 2. Denmark, 2021.
3. Federal Law "On State Regulation of Production and Turnover of Ethyl alcohol, Alcoholic and Alcohol-containing Products and on Restriction of Consumption (Drinking) of Alcoholic Beve­rages" dated 22.11.1995 N 171-FZ. (In Russ.).
4. Ermolaeva G.A. (Ed.). Directory of the employee of the laboratory of the brewing enterprise. Saint-Petersburg: Publishing house Profession, 2004. 535 p. (In Russ.).
S.Yu. Solov'eva, Candidate of Technical Science,
NewBio LLC, Russia
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M.D. Khlynovskii
Montebrew DOO, Montenegro

Pankratov A.A., Astakhova A.Yu., Kolganov I.M., Lopatina O.M., Dymova A.A.Dedusting Filtration of Beer and Purification of Process Gases at Breweries

P. 8-10

Miller Yu.Yu.New Scientific and Practical Approaches to the Production of Fermented Grain Drinks

P. 11-16 DOI: 10.52653/PIN.2023.03.03.003

Key words
fermented grain beverages, modular colorimetric approach, food system, beverage technology, nutritional value, biological value

In the current socio-economic conditions, the consumer market, in particular for food products, is at risk of providing products not only of imported production, but also of domestic products produced on the basis of imported raw materials. This problem also affected a group of soft drinks, the assortment of which underwent changes at the beginning of the sanctions actions towards Russia due to the withdrawal of well-known foreign brands from the market. In this regard, it is necessary to take actions for the uninterrupted supply of high-quality and safe products, which can be realized by increasing the capacity of domestic enterprises of the food and processing industry, as well as the development of the assortment of both finished products and raw materials used in its production. The paper presents new conceptual approaches to the production of fermented grain drinks such as kvass. The basic principles of the development of this group of beverages are proposed, including expanding the assortment, increasing the nutritional and biological value, uninterrupted supply of raw materials to manufacturers, etc. When forming the concept, the strategic directions of the development of the food and processing industry, the demand for and competitiveness of products, the capabilities of the domestic agro-industrial complex were taken into account, as a result of which a model for the formation of raw materials and the production of fermented grain beverages based on them was proposed. The proposed modular-algorithmic model in the technology of beverages with a preliminary goal-oriented preparation of raw materials is based on the application of traditional algorithmic actions adopted at the enterprises of the beer and alcohol industry (technological modes, equipment, etc.), and the formalization of modular elements of the system with individual trajectories depending on the type of raw materials used and the goals set in production, including product requirements (nutritional composition, compliance with regulated quality and safety indicators). For practical confirmation of the proposed concept, the possibility of obtaining fermented grain drinks based on interchangeable raw materials (barley, wheat, rye, oats and soy) and the formation of a purposefully established food system, including with increased nutritional and biological value in terms of such indicators as the content of amino acids, B vitamins and organic acids, has been experimentally tested.

1. Agafonov GV, Chusova AE, Zelenkova AV, Plotnikov VE. Effect of enzyme preparation Ceramics 6xmg on indicators of oat malt quality. Vestnik Voronezhskogo gosudarstvennogo universiteta inzhenernyh tekhnologij = Bulletin of the Voronezh State University of Engineering Technologies. 2018; (3(77)): 128-133. https://doi.org/10.20914/2310-1202-2018-3-128-133. (In Russ.).
2. Katsurba TV, Evstaf'ev SN, Frantenko VK, Demina AI. Sodium selenite as an intensifier in the malting of brewing barley. Izvestiya vuzov. Prikladnaya himiya i biotekhnologiya = News of universities. Applied Chemistry and Biotechnology. 2018;8(1(24)): 67-73. https://doi.org/10.21285/2227-2925-2018-8-1-67-73. (In Russ.).
3. Kiseleva TF, Grebennikova YuV, Reznichenko IYu, Miller YuYu, Vereshchagin AL. Research of the possibility of using an organic stimulant in the production of non-traditio­nal malts. Pishchevaya promyshlennost' = Food industry. 2019; (10):32-36. https://doi.org/
10.24411/0235-2486-2019-10153. (In Russ.).
4. Miller YuYu, Kiseleva TF. Biotechnological approach to the intensification of soy malt production. Biotekhnologiya = Biotechnology. 2022;38(6):84-89. https://doi.org/10.56304/S0234275822060096. (In Russ.).
5. Miller YuYu, Kiseleva TF, Permyakova LV, Arysheva YuV. The effect of inorganic processing during malting on the enzymatic activity of wheat malt. Pishchevaya promyshlennost' = Food industry. 2022;(1):42-45. https://doi.org/10.52653/PPI.2022.1.1.009. (In Russ.).
6. Miller YuYu, Kiseleva TF, Pomozova VA. Intensification of rye malting using the me­thod of enzymatic catalysis. Pishchevaya promyshlennost' = Food industry. 2023;(5):81-83. https://doi.org/10.52653/PPI.2023.5.5.023. (In Russ.).
7. Mukailov MD, Hokonova MB. A way to improve the quality of malt. Problemy razvitiya APK regiona = Problems of agro-industrial complex development in the region. 2018; (3(35)): 181-184. (In Russ.).
8. Rostovskaya MF, Boyarova MD, Klykov AG. Effect of various barley steeping conditions on the content of albuminous substances in the malt. Tekhnika i tekhnologiya pishchevyh proizvodstv = Food processing: techniques and technology. 2020;50(2):319-328. https://doi.org/10.21603/2074-9414-2020-2-319-328. (In Russ.).
9. Khokonova MB. Application of enzyme prepa­rations in the production brewing malt. Izvestiya Kabardino-Balkarskogo gosudarstvennogo agrarnogo universiteta im. V.M. Kokova = Proceedings of Kabardino-Balkarian State Agrarian University named after V.M. Kokov. 2016;(1(11)):50-54. (In Russ.).
10. Chanchikova AA, Kamenskaya EP. Research of influence of enzyme drugs on indicators of quality of light barley malten. Tekhnolo­giya i tovarovedenie innovacionnyh pishchevyh produktov = Technology and merchandising of the innovative foodstuff. 2020;(5(64)):17-22. https://doi.org/10.33979/2219-8466-2020-64-5-17-22. (In Russ.).
11. Shepshelev AA, Kulikov AV, Litvinchuk AA, Danilyuk AS. Intensification of malt on the basis of biostimulation. Pishchevaya promyshlennost': nauka i tekhnologii = Food industry: science and technology. 2019;12(4(46)): 53-58. (In Russ.).
12. Agu RC, Okeke BC. Effect of potassium bromate on diastase, cellulase and hemicellulase development in Nigerian malted millet (Pennisetum maiwa). Process Biochemistry. 1992;27:335-338.
13. Basinci F, Mogol BA, Guler S, Gokmen V, Koksel H. Mitigation of acrylamide formation du­ring malt processing. Journal of Cereal Science. 2022;106:103485. https://doi.org/10.1016/j.jcs.2022.103485.
14. Kalita D, Sarma B, Srivastava B. Influence of germination conditions on malting potential of low and normal amylose paddy and changes in enzymatic activety and hysic chemical properties. Food Chemistry. 2017;220:67-75. https://doi.org/10.1016/j.foodchem.2016.09.193.
15. Kamenskaia EP, Obrezkova MV. Using probio­tic microorganisms in kvass technology fermentation. Tekhnologiya i tovarovedenie innovacionnyh pishchevyh produktov = Technology and merchandising of the innovative foodstuff. 2015;(6(35)): 24-30. (In Russ.).
16. Pomozova VA, Kiseleva TF, Zarubina AA, Zarubin DA. Comparative evaluation of the quality of dry baking yeast for kvass production. Pivo i napitki = Beer and beverages. 2008;(2):58-61. (In Russ.).
17. Sergeeva IYu, Unschikova TA, Rysina VYu. Ways of improvement fermented kvass technology based on the analysis of modern scientific and technical developments. Tekhnika i tekhnologiya pishchevyh proizvodstv = Food processing: techniques and technology. 2014;(3(34)): 69-78. (In Russ.).
18. Kiseleva TF, Grebennikova YV, Miller YY, Golub OV, Bakaytis VI. The elaboration of the technology of polymalt beverages recommended to the population of kemerovo region under the conditions of negative ecological environment. IOP Conference Series: Earth and Environmental Science. Current Problems and Solutions. 2019;224(1):012002. https://doi.org/10.1088/1755-1315/224/1/012002.
Yulia Yu. Miller, Candidate of Technical Science, Associate Professor,
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Siberian University of Consumer Cooperation,
26, K. Marx Avenue, Novosibirsk, 630087, Russia


Mikhailova I.Yu., Sinelnikova M.Yu. New Technologies and Raw Materials in the Production of Vegetable Drinks

P. 17-21 DOI: 10.52653/PIN.2023.03.03.005

Key words
plant-based drinks, probiotics, fermentation, double emulsions, microcellulose

The desire of modern society for a balanced and varied diet, for a healthy lifestyle, the popularity of various types of diets and at the same time the increase in the prevalence of lactose intolerance, as well as the rejection of animal products for various reasons, has become an impetus for the development of alternative foods that are no less valuable in their nutritional properties. The last decade has been a breakthrough in the search for new sources of raw materials and the expansion of the range of finished products, both mixed and completely plant-based. For the production of vegetable analogues of milk, traditional technologies are more often used, including such stages as soaking, grinding, aging, extraction, filtration, homogenization, etc. their basis, meeting the requirements of a balanced chemical composition and food security. This is the use and germination of the original grain and leguminous raw materials in order to enrich it with compounds released by grain during its growth activation and enzymatic hydrolysis during growth; and fermentation of the plant base with fermented milk cultures, previously used for ordinary milk and enhancing the beneficial properties of the plant component by the action of probiotics; improving the stability of the colloidal system to reduce segregation during storage; the use of double encapsulation to introduce missing microelements and vitamins into the composition of vegetable drinks, which increase the value without the use of additional chemical ingredients and retain labile ingredients at all stages of the technological process. Technologies associated with exposure to ultraviolet radiation, ultrasound, ohmic heating, pulsed electric field and high pressure allow not only to better preserve the beneficial properties of plant materials, inactivate endogenous enzymes, reduce pathogenic microbial load, but also increase the nutritional value of the product. The choice of a technological approach is also focused on maintaining the colloidal stability of the vegetable milk replacer and improving the organoleptic characteristics of the product.

1. Vafin RR, Mikhailova IY, Ageykina II, Kharlamova LN. Modeling of DNA technology for species identification of the raw composition of plant-based beve­rages. Pishchevaya promyshlennost' = Food industry. 2023;(8):107-111. https://doi.org/10.52653/PPI.2023.8.8.020. (In Russ.).
2. Moskovenko NV. Issledovanie pokazatelei kachestva rastitel'nykh napitkov molochnogo tipa. [Study of indicators of the qua­lity of dairy-type vegetable drinks]. Quality of pro­ducts, technologies and education. Materials of the XVI International Scientific and Practical Conference. Magnitogorsk, 2021, pp. 36-41. (In Russ.).
3. Medvedev OS, Medvedeva NA. Vegetable milk substitutes: features, benefits, use in nutrition. Voprosy dietologii = Problems of Dietology. 2018;8(1):52-58. https://doi.org/10.20953/2224-5448-2018-1-52-58. (In Russ.).
4. Furik AO. Awareness of the population on the nutritional value of animal milk and dairy products of vegetable origin. Sovremennye problemy gigieny, radiatsionnoi i ekologicheskoi meditsiny = Modern problems of hygiene, radiation and environmental medicine. 2022;12(5):247-258. (In Russ.).
5. Merenkova SP, Androsova NV. Topical aspects of producing beverages based on plant raw material. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i biotekhnologii = Bulletin of the South Ural State University. Series: Food and biotechnologies. 2018;6(3):57-67. https://doi.org/10.14529/food180307. (In Russ.).
6. Merenkova SP, Rezanova MA. Technological aspects of producing fermented beverages with antioxidant properties based on grain raw materials. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i biotekhnologii = Bulletin of the South Ural State University. Series: Food and biotechnologies. 2022;10(1):76-85. https://doi.org/10.14529/food220109. (In Russ.).
7. Khrundin DV, Miassarova DM. Plant-based fermented product manufacture using lactic acid bacteria cultures. Industriya pitaniya = Nutrition Industry. 2022;7(4):59-66. https://doi.org/10.29141/2500-1922-2022-7-4-7. (In Russ.).
8. Ionova KS, Bakumenko OE, Bakumenko PV. Development of technology for a functional grain-based drink. Khranenie i pererabotka sel'khozsyr'ya = Storage and processing of agricultural raw materials. 2022;(4):164-179. https://doi.org/10.36107/spfp.2022.293. (In Russ.).
9. Serazetdinova YuR, Frolova AS, Milentyeva IS, Minina VI. Study of the ability of lactic acid bacteria to ferment plant analogues of milk. XXI vek: itogi proshlogo i problemy nastoyashchego plyus = XXI century: results of the past and problems of the present plus. 2022;11(3(59)):128-134. https://doi.org/10.46548/21vek-2022-1159-0019. (In Russ.).
10. Popova NV, Kalinina IV. Study of the kine­tics of the fermentation process of soy milk by starter culture Lactobacillus Acidophilus. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i biotekhnologii = Bulletin of the South Ural State University. Series: Food and biotechnologies. 2023;11(2):74-82. https://doi.org/ 10.14529/food230209. (In Russ.).
11. Shirobokova OL, Lepekhina EV. Fermentation of vegetable milk. Khimiya. Ekologiya. Urbanistika = Chemistry. Ecology. Urbanistics. 2021;2:210-213. (In Russ.).
12. Shirobokova OL, Portnova AV, Lepekhina EV. Determination of the amount of lactic acid microorganisms in the fermentation pro­duct of vegetable milk. Khimiya. Ekologiya. Urbanistika = Chemistry. Ecology. Urbanistics. 2022;2:162-166. (In Russ.).
13. Kustova OS, Vladimirova EP. Prospects for the development of vegetable milk types. Akademicheskaya publitsistika = Academic journalism. 2021;(11-2):18-21. (In Russ.).
14. Feofilaktova OV, Zavorokhina NV. Modeling of a plant-based milk analogue with increased nutritional value. Tekhnologii pishchevoi i pererabatyvayushchei promyshlennosti. APK-produkty zdorovogo pitaniya = Technologies of the food and processing industry. APK-pro­ducts of healthy food. 2022;(4):31-38. https://doi.org/10.24412/2311-6447-2022-4-31-38. (In Russ.).
15. Shishkina DI, Shtovhun AI, Klein EE., Berketova LV. Modern technologies for the production of alternative milk from plant products. Vestnik VGUIT = Proceedings of the Voronezh state university of engineering technologies. 2022;84(4(94)):141-148. https://doi.org/10.20914/2310-1202-2022-4-141-148. (In Russ.).
16. Kalinina IV, Naumenko NV, Uday B, Kadi AMY, Malinin AV, Tsaturov AV. Possibilities of use of double emulsions in the food industry. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i biotekhnologii = Bulletin of the South Ural State University. Series: Food and biotechnologies. 2022;10(4):109-114. https://doi.org/10.14529/food220411. (In Russ.).
17. Possas A, Valero A, Garcia-Gimeno RM, Perez-Rodriguez F, Souza PM. Influence of temperature on the inactivation kinetics of Salmonella Enteritidis by the application of UV-C technology in soymilk. Food Control. 2018;94:132-139. https://doi.org/10.1016/j.foodcont.2018.06.033.
18. Atuonwu JC, Leadley C, Bosman A, Tassou SA. High-pressure processing, microwave, ohmic, and conventional thermal pasteurization: quality aspects and energy economics. Journal of Food Process Engineering. 2020;43(2):e13328. https://doi.org/10.1111/jfpe.13328.
19. Merenkova SP, Zinina OV. Study of the structure and microbiologicalindicators of fermented plant beverages. Polzunovskii vestnik = Polzunovskiy vestnik. 2023;(1):59-66. https://doi.org/10.25712/ASTU.2072-8921.2023.01.008. (In Russ.).
20. Myachikova NI, Sorokopudov VN, Binkovskaya OV, Dumacheva EV. Phenological rhythms of plants this. Rosaceae juss. Naturally central russian upland. Sovremennye problemy nauki i obrazovaniya = Modern problems of science and education. 2012;(5):103. (In Russ.).
21. Popova NV, Gavrilova KS, Naumenko EE. Evaluation of the possibility of using sprou­ted green buckwheat in the technology of probiotic drinks. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Pishchevye i biotekhnologii = Bulletin of the South Ural State University. Series: Food and biotechnologies. 2022;10(4):18-25. https://doi.org/10.14529/food220402. (In Russ.).
22. Zenkova ML, Melnikova LA, Timofeeva VN. Non-alcoholic beverages from sprouted buckwheat: technology and nutritional value. Tekhnika i tekhnologiya pishchevykh proizvodstv = Technique and technology of food production. 2023;53(2):316-325. https://doi.org/10.21603/2074-9414-2023-2-2435. (In Russ.).
Irina Y. Mikhailova,
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Marina Y. Sinelnikova,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow,119021, Russia


Tomgorova S.M., Trofimchenko V.A. The Effect of Visible Light on the Change in the Composition of Extractive Substances of White Sparkling Wines

P. 22-27 DOI: 10.52653/PIN.2023.03.03.004

Key words
sparkling wine, storage conditions, visible light, physico-chemical composition, organic acids, glycerin, amino acids

Preserving the quality and safety of bottled sparkling wine is a fundamental aspect, both for producers of this type of product and for consumers. As is known, exposure to high temperatures and natural light during the storage of finished products contributes to an increase in the intensity of oxidative processes taking place in it, which provokes a decrease in its consumer properties. In this regard, studies aimed at establishing optimal storage conditions for sparkling wines in consumer packaging are relevant. The purpose of this work was to study the effect of visible light in the middle range of the spectrum on the physico-chemical parameters of sparkling wines. Objects of research: white sparkling brut wines, bottled in glass bottles of dark olive color. Control samples were stored in the dark and under natural light, experimental samples were stored in specially equipped chambers with artificial lighting emitting various wavelengths: l = 440-485 nm (blue light), l = 500-565 nm (green light) and l = 565-590 nm (yellow light). The mass concentration of organic acids, sugars, glycerol and amino acids was determined in the initial samples, as well as control and experimental samples after 30 and 60 days of storage. A slight decrease in glycerin was observed in samples stored in natural light and under the influence of blue light. A similar pattern was also observed in the change in the mass concentration of phenylalanine, threonine, tryptophan, tyrosine and methionine - amino acids that affect the aroma of sparkling wine. There was also a change in the mass concentration of organic acids in these samples. Thus, the data obtained, as well as the results of the organoleptic evaluation, allow us to conclude that storing white sparkling brut wines in the dark or using green or yellow light will contribute to the maximum preservation of the original quality of the finished product.

1. Arena E, Rizzo V, Licciardello F, Fallico B, Muratore G. Effects of light exposure, bottle colour and storage temperature on the quality of Malvasia delle lipari sweet wine. Foods. 2021;10(8):1881. https://doi.org/10.3390/foods10081881.
2. Benucci I. Impact of post-bottling storage conditions on colour and sensory profile of a ros? sparkling wine. Leben­smittel-Wissenschaft und-Technologie. 2019;118:108732. https://doi.org/10.1016/j.lwt.2019.108732.
3. Grant-Preece P, Barril C, Schmidtke LM, Scollary GR, Clar AC. Light-induced changes in bottled white wine and underlying photochemical mechanisms. Critical Reviews In Food Science and Nutrition. 2017;57(4):743-754. https://doi.org/10.1080/10408398.2014.919246.
4. Thompson-Witrick KA, Pitts ER, Nemenyi JL, Budner D. The impact packaging type has on the flavor of wine. Beverages. 2021;7(2):36. https://doi.org/10.3390/beverages7020036.
5. CHemisova LE, Ageeva NM, YAkimenko EN. The quality and safety of products during storage in the "wine - packaging, capping" system. Tekhnika i tekhnologiya pishchevykh proizvodstv = Technique and technology of food production. 2023;53(2):281-293. https://doi.org/10.21603/2074-9414-2023-2-2432. (In Russ.).
6. Ghidossi R, Poupot C, Thibon C, Pons A, Darriet P, Riquier L, Revel GDe, Mietton-Peuchot M. The influence of packaging on wine conservation. Food Control. 2012;23(2):302-311. https://doi.org/10.1016/j.foodcont.2011.06.003.
7. Dozon NM, Noble A. Sensory study of the effect of fluorescent light on a sparkling wine and its base wine. American Journal of Enology and Viticulture. 1989;40(4):265-271. https://doi.org/10.5344/ajev.1989.40.4.265.
8. Caceres-Mella A, Flores-Valdivia D, Laurie VF, Lopez-Solis R, Pena-Neira A. Chemical and Sensory Effects of Sto­ring Sauvignon Blanc Wine in Colored Bottles under Artificial Light. Journal of Agricultural and Food Chemistry. 2014;62(29):7255-7262. https://doi.org/10.1021/jf501467f.
9. Celotti E, Lazaridis G, Figelj J, Scutaru Y, Natolino A. Comparison of a rapid light-induced and forced test to study the oxidative stability of white wines. Mo­lecules. 2022;27(1):326. https://doi.org/10.3390/molecules27010326.
10. Moriones J, Jimenez-Moreno N, Ancin-Azpilicueta C, Fernandez de Ara J, Navarcorena B, Almandoz E, Esparza I. Development of an irradiation equipment to accelerate the degradation of ros? wine in antique green and flint bottles. Current Research in Food Science. 2023;6:100501. https://doi.org/10.1016/j.crfs.2023.100501.
11. Guerrini L, Pantani OL, Politi S, Angeloni G, Masella P, Calamai L, Parenti A. Does bottle color protect red wine from photo-oxidation? Packaging Technology and Science. 2019;32(5):259-265. https://doi.org/10.1002/pts.2433.
12. Diaz-Maroto MC, Vinas ML, Marchante L, Alanon ME, Diaz-Maroto IJ, Perez-Coello MS. Evaluation of the sto­rage conditions and type of cork stopper on the quality of bottled white wines. Mo­lecules. 2021;26(1):232. https://doi.org/10.3390/molecules26010232.
13. Avakyanc SP. Igristye vina [Sparkling wines]. Moscow: Agropromizdat, 1986. 272 p. (In Russ.).
14. Kishkovskij ZN, Merzhanian IM. Himiya vina [Chemistry of wine] Moscow: Agropromizdat, 1988. 254 ð. (In Russ.).
15. Oganesyanc LA, Peschanskaya VA, Dubinina EV. Improving the quality assessment of table wine materials for sparkling wines. Pivo i napitki = Beer and beverages. 2018;(3):72-75. (In Russ.).
16. Lutkov IP, Makorov AS, ZHilyakova TA, Aristova NI, Bilyaeva VI, Panova EP, Psutudi DT. Issledovanie osnovnyh organicheskih kislot v vinomateralah dlya igristyh vin sevastopol'skoj zony [Research of basic organic acids in wine materials for sparkling wines of the Sevastopol zone]. Uchenye zapiski Tavricheskogo nacional'nogo universiteta im. V.I. Vernadskogo. Seriya "Biologiya, himiya" = Scientific notes of V.I. Vernadsky Tauride National University. Series "Biology, Chemistry". 2007:20(4):144-151. (In Russ.).
17. Clark AC, Dias DA, Smith TA, Ghiggino KP, Scollary GR. Iron (III) tartrate as a potential precursor of light-induced oxidative degradation of white wine: studies in a model wine system. Journal of Agricultu­ral and Food Chemistry. 2011;59(8):3575-3581. https://doi.org/10.1021/jf104897.
18. Lisovec UA, Ageeva NM, SHirshova AA. Changes in the quantative composition of amino acids during batonnage in the technology of white table wines. Politematicheskii setevoi elektronnyi nauchnyi zhurnal KubGAU = Polythematic online scientific journal of Kuban state agrarian university. 2016;120:478-487. (In Russ.).
19. Dias DA, Smith TA, Ghiggino KP, Scollary GR. The role of light, temperature and wine bottle colour on pigment enhancement in white wine. Food Chemistry. 2012;135(4):2934-2941. https://doi.org/10.1016/j.foodchem.2012.07.068.
Svetlana M. Tomgorova, Candidate of Technical Science,
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Vladimir A. Trofimchenko, Candidate of Technical Science,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow,119021, Russia


Gribkova I.N.The Physical Treatment Influence on Brewer's Spent Grain Extract Phenolic Composition

P. 28-32 DOI: 10.52653/PIN.2023.03.03.002

Key words
brewer's spent grain, physical treatment, ECA-water, brewer's spent grain structure, phenolic compounds

The article deals with the environmentally friendly processing issue the brewer's spent grain structure. The brewer's spent grain chemical composition is presented, in which various fractions of phenolic compounds occupy a significant place. The goal set by the authors - to study the influence of environmental methods of processing brewer's spent grain on the phenolic compounds extraction was solved using research methods accepted in the industry. It has been shown that the presence of a catholyte and various physical treatments have different effects on the dimeric and monomeric forms of bound phenolic compounds. The authors studied the change in the content of phenolic compound dimeric forms (catechins, rutin and quercetin). Studies have shown that after the first stage of the 3-stage treatment, catechins and rutin are most effectively extracted with catholyte. It has been shown that treatment at atmospheric pressure and 60 °C is the most optimal, since the catechins concentration in extracts ranges from 0.24-0.3 mg/g of grain, and rutin - 0.38-0.83 mg/g of grain, respectively and these concentration indicators are the largest of all the studied types of treatment. The excess pressure (0.5-1.0 atm) use at stage 1 leads to the catechins oxidation by 50%, and during sonication, the catechins loss is 12-42%. It was shown that gallic acid, vanillin and lilac aldehyde were the most significant in the content of extracts monomeric forms. Along with this, it was noted that there was a quantitative increase in the aldehyde forms of phenolic compounds during the processing of brewer's spent grain in a catholyte with excess pressure. It has been shown that catechins are the most labile among the dimeric forms, since they are not detected in 4% water-alcohol extracts of completely processed brewer's spent grain. Rutin is extracted the most, and an increase in the content is directly proportional to the extraction duration - an increase in the duration of treatment at stage 1 by 3 h contributes to an increase in the rutin content by a factor of 2.4 compared with treatment at atmospheric pressure for 3 h. The authors showed that under conditions of excessive pressure stage 1 processing, that is, both high temperature and excess pressure, contribute to the rutin oxidation. Its content drops to 0.003 mg/g brewer's spent grain, regardless of the conditions. The impact of ultrasonic treatment increases the rutin content in the extracts by 3 times compared to the exposure conditions to excess pressure. The authors have shown that vanillin is the most significant in hydroalcoholic extracts, and its content depends on the type of physical treatment.

1. Mitri S, Salameh S-J, Khelfa A, Leonard E, Maroun RG, Louka N, Koubaa M. Valorization of Brewers' Spent Grains: Pretreatments and Fermentation, a Review. Fermentation. 2022;8(2):50. https://doi.org/10.3390/fermentation8020050.
2. Agrawal D, Gopaliya D, Willoughby N, Khare SK, Kuymare V. Recycling potential of brewer's spent grains for circular biorefineries. Current Opinion in Green and Sustainable Chemistry. 2023;40:100748. https://doi.org/10.1016/j.cogsc.2022.100748.
3. Cooray ST, Chen WN. Valorization of brewer's spent grain using fungi solid-state fermentation to enhance nutritional value. Journal of Functional Foods. 2018;42:85-94. https://doi.org/10.1016/j.jff.2017.12.027.
4. Kavalopoulos M, Stoumpou V, Christofi A, Mai S, Barampouti EM, Moustakas K, Malamis D, Loizidou M. Sustainable valorisation pathways mitigating environmental pollution from brewers' spent grains. Environmental Pollution. 2021;270:116069. https://doi.org/10.1016/j.envpol.2020.116069.
5. Forssell P, Kontkanen H, Schols H, Hinz S, Vincent G, Buchert J. Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes. Journal of the Institute of Brewing. 2008;114(4):306-314. https://doi.org/10.1002/j.2050-0416.2008.tb00774.x.
6. Lisci S, Tronci S, Grosso M, Karring H, Hajrizaj R, Errico M. Brewer's Spent Grain: Its Value as Renewable Biomass and Its Possible Applications. Chemical Engineering Transactions. 2022;92:259-264. https://doi.org/10.3303/CET2292044.
7. Ikram S, Huang L, Zhang H, Wang J, Yin M. Composition and Nutrient Value Proposition of Brewers Spent Grain. Journal of Food Science. 2017;82(10):2232-2242. https://doi.org/10.1111/1750-3841.13794.
8. Chetrariu A, Dabija A. Brewer's Spent Grains: Possibilities of Valorization, a Review. Applied Sciences. 2020;10(16):5619. https://doi.org/10.3390/app10165619.
9. Cooray ST, Lee JJL, Chen WN. Evaluation of brewers' spent grain as a novel media for yeast growth. AMB Express. 2017;7:117. https://doi.org/10.1186/s13568-017-0414-1.
10. Verni M, Pontonio E, Krona A, Jacob S, Pinto D, Rinaldi F, Verardo V, D?az-de-Cerio E, Coda R, Rizzello CG. Bioprocessing of Brewers' Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides. Frontiers in Microbiology. 2020;11:1831. https://doi.org/10.3389/fmicb.2020.01831.
11. Silva MC, dos Anjos JP, Guarieiro LLN, Machado BAS. A Simple Method for Evaluating the Bioactive Phenolic Compounds' Presence in Brazilian Craft Beers. Molecules. 2021;26(16):4716. https:// doi.org/10.3390/molecules26164716.
12. Salawu SO, Olukemi BE, Asikhia IC, Akindahunsi AA. Mineral elements bio-accessibility and antioxidant indices of blanched basella rubra at different phases of in vitro gastrointestinal digestion. Preventive nutrition and food science. 2018;23(1):22-29. https://doi.org/10.3746/pnf.2018.23.1.22.
13. Kobelev KV, Gernet MV, Gribkova IN. Razrabotka innovatsionnogo sposoba polucheniya biologicheski aktivnykh soyedineniy pivnoy drobiny [Development of an innovative method for obtaining biologically active compounds of brewer's spent grain] // Tekhnika i tekhnologiya pishchevykh proizvodstv = Technique and technology of food production. 2021;51(1):113-124. https://doi.org/10.21603/2074-9414-2021-1-113-124. (In Russ.).
14. Danylkovych AG, Lishchuk VI, Romaniuk OO. Use of electrochemically activated aqueous solutions in the manufacture of fur materials. SpringerPlus. 2016;5:214. https://doi.org/10.1186/s40064-016-1784-6.
15. Jiang H, Xue A, Wang Z. Electrochemical degradation of lignin by ROS. Sustai­nable Chemistry. 2020;1(3):345-360. https://doi.org/10.3390/suschem1030023.
16. Coelho E, Rocha MAM, Saraiva JA, Coimbra MA. Microwave superheated water and dilute alkali extraction of brewers' spent grain arabinoxylans and arabinoxylo-oligosaccharides. Carbohydrate Polymers. 2014;99:415-422. https://doi.org/10.1016/j.carbpol.2013.09.003.
Irina N. Gribkova, Candidate of Technical Science,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7, Rossolimo Str., Moscow,119021, Russia

Panasyuk A.L., Beloslyudova G.Yu., Klepikov D.V.Reduction of Residual Amounts of Some Pesticides in Fruit Fermented Materials

P. 33-37 DOI: 10.52653/PIN.2023.03.03.006

Key words
fermented fruit materials, pesticides, deltamethrin, malathion, zirconium hydrophosphate, Thermoxide-3A

The possibility of reducing the residual content of pesticides in fermented fruit materials by processing preparations based on zirconium hydrophosphate was investigated. Fruit juices, ciders, fermented fruit materials, as well as alcoholic fruit products (fruit wines) may contain a certain amount of pesticides, as well as products of their destruction. The most common phosphorus- and chlorine-containing pesticides have a long decay period, in this regard, in recent years, drugs that decompose rapidly in the soil have been used. These include, in particular, deltamethrin, which is a pyrethroid essential insecticide, as well as malathion, which is an insecticide and acaricide of a wide spectrum of action. Although malathion belongs to the class of organophosphorus compounds, it has a mild toxicity compared to other pesticides of the same nature. The presence of residual pesticides in natural fruit juices is more likely than the fermentation products obtained from them (ciders, etc.), since yeast sorbs a significant part of pesticides on itself. Ensuring the safety of fruit products is achieved primarily by preventive measures related primarily to the strict dosage of the drug when applied to the soil, washing fruits, etc. However, if there are residual amounts of pesticides in the products, their removal is a problem. A method for removing pesticides from fruit processing products using the preparation Thermoxide-3A, produced on the basis of zirconium hydrophosphate, is proposed. This preparation is intended for sorption of heavy metal cations from liquid products. However, its high adsorption properties have revealed the possibility of removing pesticides such as deltamethrin and malathion from products. The treatment modes of fermented fruit materials with this preparation are proposed.

1. Soldatenkov AT, Kolyadina NM, Le Tuan A. Pestitsidy i regulyatory rosta: prikladnaya i organicheskaya khimiya. [Pesticides and growth regulators: Applied and organic chemistry]. Moscow: Binom. Laboratoriya znanii, 2013. 223 p. (In Russ.).
2. Gerunov TV, Redkin YuV, Gerunova LK. Immunotoxicity of pesticides and its role in animal and human pathology. Uspekhi sovremennoi biologii = Successes of modern biology. 2011;131(5):474?482.
3. Chiburayev VI, Dvoskin YaG, Bragina IV, Ivanov AA, Garbuzova AA. Pollution of the territory of the russian federation with pesticides as a potential danger to people's health. Gigiena i sanitariya = Hygiene and sanitation, russian journal. 2003;(3):68-71. (In Russ.).
4. Zav'yalova N. Traces of "chemistry". Which products contain the most pesticides? [Internet]. [cited 2022 June 14]. URL: https://rskrf.ru/tips/obzory-i-topy/sledy-khimii-v-kakikh-produktakh-bolshe-vsego-pestitsidov/?ysclid=lmg3zun6hk622428668 (In Russ.).
5. Zharkova IM, Malyutina TN. Mediko-biologicheskie trebovaniya sanitarnye normy kachestva rastitel'nogo syr'ya i pishchevykh produktov. [Medical and biological requirements sanitary standards of quality of plant raw materials and food products]. Voronezh: Voronezhskii gosudarstvennyi universitet inzhenernykh tekhnologii, 2017. 224 p. (In Russ.).
6. Sarishvili NG, Panasyuk AL, Kuzmina EI, Belova LN, Persianov VI. Transformatsiya pestitsidov v pochve vinogradnikov. [Transformation of pesticides in the soil of vineyards]. Vinograd i vino Rossii = Grapes and wine of Russia. 2000;(5):17-20. (In Russ.).
7. Sarishvili NG, Panasyuk AL, Kuzmina EI, Belova LN. Migratsiya khlorsoderzhashchikh pestitsidov v produktakh pererabotki vinograda. [Migration of chlorine-containing pesticides in grape processing products]. Khranenie i pererabotka sel'khozsyr'ya = Storage and processing of farm products. 1999;(9):49-51. (In Russ.).
8. Panasyuk AL, Kuzmina EI, Kharlamova LN, Babaeva MV, Romanova IP, Shichiyakh RA. Study of the effect of organochlorine pesticides on biochemical transformations in the process of obtaining grape mate­rials. International journal of pharmaceutical research. 2020;12(4):1788-1796. https://doi.org/10.31838/ijpr/2020.12.04.255.
9. Sarishvili N. G., Panasyuk A.L., Stolyarova E.I., Gulevskaya S.A. The effect of pesticides on physiological and morphological changes and yeast metabolism in the production of fruit wines. Vinograd i vino Rossii = Grapes and wine of Russia. 1995;(6):25. (In Russ.).
10. Panasyuk AL, Kuzmina EI, Kharlamova LN, Babaeva MV, Romanova IP. Influence of organochlorine pesticides on biochemical transformations in the process of obtaining apple wine materials. IOP Conference Series: Earth and Environmental Science. 2021;666:052004. https://doi.org/10.1088/1755-1315/666/5/052004.
11. Ageeva NM, Kovalenko OA, Guguchkina TI. Sposob udaleniya pestitsidov iz susla, ili soka, ili vina. [A method for removing pesticides from wort, or juice, or wine]. Russia patent SU 1759869 A1. 1992.
12. Ageeva NM, Guguchkina TI, Kosenko MM, Markovskii MG. Sposob udaleniya pestitsidov iz vinodel'cheskoi produktsii. [Method of removing pesticides from wine products]. Russia patent RU 2406755. 2009.
13. Ageeva NM, Markovskiy MG, Antonenko MV. Thermoxid-3a for stabilization of wines to crystal turbid. Plodovodstvo i vinogradarstvo Yuga Rossii = Fruit growing and viticulture in the South of Russia. 2020;(63(3)):206-216. https://doi.org/10.30679/2219-5335-2020-3-63-206-216. (In Russ.).
14. Ismail MF, Mohamed HM. Modulatory effect of lycopene on deltamethrin-induced testicular injury in rats. Cell biochemistry and biophysics. 2013;65(3):425-432. https://doi.org/10.1007/s12013-012-9446-y.
15. Gerunov TV, Chigrinskii EA, Fedorov YuN, Gerunova LK, Konvai VD. Adaptive and compensatory responses in rats at the early stages of an acute intoxication with deltamethrin. Sel'skokhozyaistvennaya biologiya = Agricultural biology. 2016;51(4):516-523. https://doi.org/10.15389/agrobiology.2016.4.516rus. (In Russ.).
16. Dorozhkin VI, Chigrinski EA, Gerunov TV. Influence of synthetic pyrethroid deltamethrinon the activity of glutathione-dependent enzymes in rat testes. Rossiiskii zhurnal problemy veterinarnoi sanitarii, gigieny i ekologii = Russian journal problems of veterinary sanitation, hygiene and ecology. 2017;(1(21)):85-90. (In Russ.).
17. Anuchina A.V. The toxic effects of pesticides on humans and animals. Mezhdunarodnyi studencheskii nauchnyi vestnik = International Student Scientific Bulletin. 2019;(1):1. (In Russ.).
18. Yurchenko VV. Long-term effects of exposure to some organophosphate pesticides. RET-INFO. 2005;(2(54)): 40-44. (In Russ.).
19. Chigrinski EA, Conway VD, Gerunova LK, Gerunov TV. Glutathione-related enzyme activity in rats' testes and epididymis at an acute intoxication with a synthetic pyrethroid deltamethrin. International Journal of Pharma and Bio Sciences. 2015;6(4):B340-B344.
20. Zinchenko VA. Khimicheskaya zashchita rastenii: sredstva, tekhnologiya i ekologicheskaya bezopasnost'. [Chemical plant protection: means, technology and environmental safety]. Moscow: KolosS, 2005. 231 p. (In Russ.).
21. Ivanov AV, Boev YuG, Egorov VI, Galyautdinova GG. Sinteticheskie piretroidy: opasnost', profilaktika i lechenie otravlenii zhivotnykh. [Synthetic pyrethroids: danger, prevention and treatment of animal poisoning]. Sostoyanie i problemy veterinarnoi sanitarii, gigieny i ekologii v zhivotnovodstve: materialy Mezhdunarodnoi nauchno-prakticheskoi konferentsii. Cheboksary, 2004. (In Russ.).
22. Kharchenko OA, Balan GM, Bubalo NN. Acute organophosphate poisoning: the main clinical syndromes and mechanisms of their formation. Sovremennye problemy toksikologii = Modern problems of toxicology. 2013;(1-2):17-31. (In Russ.).
Aleksandr L. Panasyuk, Doctor of Technical Science, Professor, Ñorresponding member of RAS,
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All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry - Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS,
7 Rossolimo Str., Moscow, 119021, Russia
Galina Yu. Beloslyudova,
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K.G. Razumovsky Moscow State University of Technologies and Management (the First Cossack University),
73, Zemlyanoy Val Str., Moscow, 109004, Russia
Dmitriy V. Klepikov,
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Inorganic Sorbents, JSC,
Industrial site of Beloyarsk Nuclear Power Plant, Zarechny, Sverdlovsk region, Russia, 624250


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